Detailed information of TA system TA149595

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 144609..145245
• Replicon: chromosome
• Accession: NZ_CP048536
• Organism: Agrobacterium tumefaciens strain SJ003

Toxin (Protein)

• Gene name: graT
• Uniprot ID: -
• Locus tag: FY140_RS00635
• Protein ID: WP_104679992.1
• Coordinates: 144964..145245 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: FY140_RS00630
• Protein ID: WP_020009806.1
• Coordinates: 144609..144908 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FY140_RS00605 (FY140_00605)	139795..140523	-	729	WP_262530764.1	ABC transporter ATP-binding protein	-
FY140_RS00610 (FY140_00610)	140520..141428	-	909	WP_262530766.1	branched-chain amino acid ABC transporter permease	-
FY140_RS00615 (FY140_00615)	141425..142297	-	873	WP_262530769.1	branched-chain amino acid ABC transporter permease	-
FY140_RS00620 (FY140_00620)	142368..143582	-	1215	WP_262530772.1	amino acid ABC transporter substrate-binding protein	-
FY140_RS00625 (FY140_00625)	143717..144589	+	873	WP_262530774.1	helix-turn-helix transcriptional regulator	-
FY140_RS00630 (FY140_00630)	144609..144908	-	300	WP_020009806.1	HigA family addiction module antitoxin	Antitoxin
FY140_RS00635 (FY140_00635)	144964..145245	-	282	WP_104679992.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FY140_RS00640 (FY140_00640)	145321..146130	-	810	WP_262530779.1	ABC transporter ATP-binding protein	-
FY140_RS00645 (FY140_00645)	146127..147161	-	1035	WP_262530781.1	iron ABC transporter permease	-
FY140_RS00650 (FY140_00650)	147164..148207	-	1044	WP_135397594.1	iron ABC transporter permease	-
FY140_RS00655 (FY140_00655)	148204..149214	-	1011	WP_135397593.1	iron-siderophore ABC transporter substrate-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (19-70)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10564.00 Da
• Isoelectric Point: 8.0066

>T149595 WP_104679992.1 NZ_CP048536:c145245-144964 [Agrobacterium tumefaciens]
MIRSFKNKLTASIDDGSVKKGFPADLVRRAQQLLTILDAATTVEDLRSPPGNRLEKLSGDREGQYSIRINKQWRICFIWT
EAGPESVEITDYH

Nucleotide

• Length: 282 bp

>T149595 NZ_CP064174:313531-314448 [Klebsiella pneumoniae]
ATGGATATACAGGCGCCTTATTATCGGCAGGTTGCTCTGCTAATGCAGGTTTTACCCTATGTTGCTGTCGAACGGGAATT
TGCGCTGAAAGGTGGCACTGCCATCAATCTCTTTATCCGCGATTTCCCTCGATTATCGGTGGATATCGACCTCGCCTGGG
TGCCCCTGGAAAGCAGAGCCATCGCTTTACCTCATATACGGGATGCGCTTGCACGTATTGCCGCCAATCTGCAACAACAG
GCAGGTATGAGCGCTGTATTACAAGCTAATCGTTCAGATGAGATGCGTGTCATTGTTACTACCGATAGCGCGCAAATAAA
GATAGAGGTTTCACCGGTCGCCCGGGGAACATTATATCCACCACAGGAGCGAGAAGTGGTGGGGGCGGTAGAGGACGTTT
TTGGCTATGCCAGCCTTCCAGTGGTTTCGTTGCCTGACCTCTACGGTGGAAAATTATGCGCGGCGCTGGATCGCCAGCAT
CCCAGAGATTTTTATGACGTGAAATTACTTCTTGATGCGCAGGAGCTTGACCGTCCCATTTTCAATGGGTTTATCGTGTA
TTTACTGAGCCATAATCGGCCTCTGGCTGAAGTGCTGAATCCTCGCTGGAAAGATATTGCTGAACCTTTTTACCGTGAAT
TCAGCGGGATGACATTTGAGACGATAGCCCTGGAGGAACTGACAGCAGTCCCCAACAGGATGATTGCTGCGTTAAAATCC
TGTTTCACACAACAGGATGTCGATTTTTTACTGTCATTTAAACGAGGCGAACCAGACTGGCGTTTAGCGCCGGAAATGCG
TATTCAGGATTTGCCTGCCGTGCAGTGGAAATTGCGCAATATTCATCAGATGCCAGCGATAAAACGCGCTGAGTCATTAG
ATAAACTGGAAAAGGTGCTGGCCGAGTGGCGCAGTTAG

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11072.92 Da
• Isoelectric Point: 8.5148

>AT149595 WP_020009806.1 NZ_CP048536:c144908-144609 [Agrobacterium tumefaciens]
MTISKSPPIHPGEILRELYLEPLGMSAYALARHLNIPRTRIERIAGEKLGITADTALRLAKFFKTTPEYWLNLQNAFDIK
AAAVRLEADLANIKELEAA

Nucleotide

• Length: 300 bp

>AT149595 NZ_CP064174:312767-313525 [Klebsiella pneumoniae]
ATGGCAACAAAATTAAACTGGCTGCTGCAAAACAGCGCTCCCGGAGATGTGATCCTTCAGTCCTGGTTAAGCCAGCATGC
CATCTCTCCCTCTCTGTCCTTTAAATACGCGCAAAGTGGCTGGTTAAAAAAAAGAGGCAACGGCGTATATGCGCGTGCGG
GACGTGAGCCGGAGTGGAACGATGCATTAGCATGTTTGCAAAATCAGCTTGCCGCTCCGGTGTATGTTGCGGGGCTCAGT
AGCTTAGTCTGGCAGGGCCGTTCACACTATCTACAGCTAAAACAAAACCAGTGCTGGCTTAGTGTGGAAAATAAAGCGCT
GCTGCCGAAATGGTTCAGGGAATTCCCGGGTGTGGAGTGGATAGTCATATCGGGGAAAAAACTGCCGGTGCTGGATGATA
GATACCGTGTAACGCTTGATATAAAAGGGAAAAAACTCATCGGCAGTGCGCCAGAACTGGCTGCTTACGAGTTGCTGAGC
GCGGTGCCGGGGACCCTCTCATTTAACCACGCTGCAGAGCTTTTCCAGGGGCTGGTTAATCTTAATCCTCGTAAAGTAGA
GTATCTTCTTTCCGTCAGCCAGTCCGTGCAGGCCAAGCGTTTGTATCTCTTCTTCGCCAGTTTTTACGAGCATGGCTGGC
TCAAACGAATCGATAGCCAAAAAATCGATCTGGGAGCGGGCAAGCGGCAAATCGTGGAAAATGGAAAGTTCAATGCTCAA
TACCAAATCACAGTGCCTGAACGCTTTCAAAAGGAATAA