Detailed information of TA system TA149561

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 352073..352669
• Replicon: plasmid pAt
• Accession: NZ_CP048471
• Organism: Agrobacterium tumefaciens strain Yub001

Toxin (Protein)

• Gene name: graT
• Uniprot ID: -
• Locus tag: FY156_RS29195
• Protein ID: WP_262529029.1
• Coordinates: 352073..352354 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FY156_RS29200
• Protein ID: WP_262528705.1
• Coordinates: 352367..352669 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FY156_RS29175 (FY156_28970)	347082..348698	-	1617	WP_262528701.1	YadA-like family protein	-
FY156_RS29180 (FY156_28975)	348658..349194	-	537	WP_262528702.1	nuclease	-
FY156_RS29185 (FY156_28980)	349206..349535	-	330	WP_262528703.1	DUF736 domain-containing protein	-
FY156_RS29190 (FY156_28985)	349584..351992	-	2409	WP_262528704.1	type IV secretory system conjugative DNA transfer family protein	-
FY156_RS29195 (FY156_28990)	352073..352354	+	282	WP_262529029.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FY156_RS29200 (FY156_28995)	352367..352669	+	303	WP_262528705.1	HigA family addiction module antitoxin	Antitoxin
FY156_RS29205 (FY156_29000)	352699..353769	-	1071	WP_262528707.1	P-type DNA transfer ATPase VirB11	-
FY156_RS29210 (FY156_29005)	353747..354964	-	1218	WP_262528709.1	type IV secretion system protein VirB10	-
FY156_RS29215 (FY156_29010)	354954..355760	-	807	WP_262528711.1	TrbG/VirB9 family P-type conjugative transfer protein	-
FY156_RS29220 (FY156_29015)	355757..356482	-	726	WP_262528712.1	type IV secretion system protein	-
FY156_RS29225 (FY156_29020)	356500..357534	-	1035	WP_262528713.1	type IV secretion system protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	htpB / htpB	1..401476	401476

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (6-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (12-70)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10545.03 Da
• Isoelectric Point: 9.9382

>T149561 WP_262529029.1 NZ_CP048471:352073-352354 [Agrobacterium tumefaciens]
MIMTSKDKRLQAVRAGKVPKGFPADILARAQRRFAQLDAAATLDDLRVPPSNNLEALPGDRKGQHSIRINGQWRICFEWR
NGDAFDVEIVDYH

Nucleotide

• Length: 282 bp

>T149561 NZ_CP064129:4431935-4432468 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11398.00 Da
• Isoelectric Point: 8.6407

>AT149561 WP_262528705.1 NZ_CP048471:352367-352669 [Agrobacterium tumefaciens]
MSNDRPRPIHPGEILREDFLSEYNLTPGALAKALLIPRDRMEKIVRGQRDITADTALRLGRYFGTTAQFWVNLQSNHDLL
VAESRFHDAIEKISPRNIAA

Nucleotide

• Length: 303 bp

>AT149561 NZ_CP064129:4431658-4431924 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA