Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3651998..3652539 Replicon chromosome
Accession NZ_CP048439
Organism Escherichia coli strain NBRC 3301

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag GXM24_RS17875 Protein ID WP_000615983.1
Coordinates 3652261..3652539 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag GXM24_RS17870 Protein ID WP_000729703.1
Coordinates 3651998..3652258 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GXM24_RS17850 3647673..3648458 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
GXM24_RS17855 3648430..3650142 + 1713 Protein_3479 flagellar biosynthesis protein FlhA -
GXM24_RS17860 3650366..3650863 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
GXM24_RS17865 3651039..3651788 - 750 WP_000056849.1 C40 family peptidase -
GXM24_RS17870 3651998..3652258 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
GXM24_RS17875 3652261..3652539 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
GXM24_RS17880 3652695..3653435 + 741 WP_001225679.1 murein L,D-transpeptidase -
GXM24_RS17885 3653406..3654173 - 768 WP_000333380.1 class II glutamine amidotransferase -
GXM24_RS17890 3654379..3654957 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - gmhA/lpcA 3602255..3676886 74631


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T149478 WP_000615983.1 NZ_CP048439:3652261-3652539 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T149478 NZ_CP064116:20720-21205 [Enterobacter hormaechei]
GTGGGACGTGTAACAGCACCAGAACCATTATCCAGCTCCCATCAACTAGCAGAGTTCGTCAGTGGTGAGGCAGTACTTGA
TGACTGGCTTAAACAGAGGGGATTAAAAAACCAAGCTCTTGGTGCAGCACGAACGTTCGTCGTTTGTAAAAGAGACACTA
AACAGGTTGTCGGTTTTTACTCTCTGGCCACCGGCAGCGTTAACCACGTGGAAGCAATGGGAAGCCTCAGGCGCAATATG
CCAGATCCAATTCCCGTAATCATACTTGCTCGCCTGGCAGTTGATGTTTCATACCGTGGCCAAGGGCTCGGGGCCGATTT
ACTGCATGATGCAGTTCTGCGCTGTTACCGTGTAGCTGAGAATATTGGAGTCCGTGCGATTATGGTTCATGCACTGACTG
AAGAAGCCAAAAACTTTTATCTTCACCATGGCTTTAAGGCATCACAAACTCAGGAACGGACATTGTTTTTAAAGTTACCA
CAGTGA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT149478 WP_000729703.1 NZ_CP048439:3651998-3652258 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT149478 NZ_CP064116:20466-20732 [Enterobacter hormaechei]
ATGAAATCAGATGTTCAGCTCAACCTTAGAGCTAAAGAATCTCAAAGAGCACTTATTGATGCTGCAGCAGAGATTCTCCA
TAAATCACGCACGGACTTCATCCTGGAAATGGCATGTCAGGCTGCGGAGAATGTGATCCTCGATCGTCGCGTATTTAATT
TTAACGATGAGCAGTATGCAGAGTTTATCGATATGCTCGATGCACCGGTTTCGGATGAGGCGGCTATTGATAAACTTCTG
GCAAGGAAACCTCAGTGGGACGTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References