Detailed information of TA system TA149477

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3645801..3646495
• Replicon: chromosome
• Accession: NZ_CP048439
• Organism: Escherichia coli strain NBRC 3301

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q47157
• Locus tag: GXM24_RS17835
• Protein ID: WP_001263489.1
• Coordinates: 3645801..3646199 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: GXM24_RS17840
• Protein ID: WP_000554758.1
• Coordinates: 3646202..3646495 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	3641389..3641469	-	81	NuclAT_12	-	-
-	3641389..3641469	-	81	NuclAT_12	-	-
-	3641389..3641469	-	81	NuclAT_12	-	-
-	3641389..3641469	-	81	NuclAT_12	-	-
GXM24_RS17810	3642065..3642523	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
GXM24_RS17815	3642784..3644241	+	1458	WP_001292994.1	cytosol nonspecific dipeptidase	-
GXM24_RS17820	3644298..3644819	-	522	Protein_3472	peptide chain release factor H	-
GXM24_RS17825	3644815..3645021	-	207	Protein_3473	RtcB family protein	-
GXM24_RS17830	3645339..3645791	-	453	WP_001059892.1	GNAT family N-acetyltransferase	-
GXM24_RS17835	3645801..3646199	-	399	WP_001263489.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
GXM24_RS17840	3646202..3646495	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
GXM24_RS17845	3646547..3647602	-	1056	WP_001226164.1	DNA polymerase IV	-
GXM24_RS17850	3647673..3648458	-	786	WP_000207552.1	putative lateral flagellar export/assembly protein LafU	-
GXM24_RS17855	3648430..3650142	+	1713	Protein_3479	flagellar biosynthesis protein FlhA	-
GXM24_RS17860	3650366..3650863	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	gmhA/lpcA	3602255..3676886	74631

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15487.84 Da
• Isoelectric Point: 7.4215

>T149477 WP_001263489.1 NZ_CP048439:c3646199-3645801 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T149477 NZ_CP064114:c8092-7589 [Enterobacter hormaechei]
ATGTTTTCAGCACAAGCCGTATACGATTTTTCGGGCTTCGACTGCGGCGAACCATCTCTGAATGATTTCCTGCAAAACCG
CCTGGTACAGCAACACAACGGGCGTATATTACGTGGCTACCTGCTTCTTACCAAAGATCCGGTGCCGAAAGTTAAGGGTT
TTTACACGCTATCCGGGAGCTGTTTTGCAAGACAGACGCTACCATCGAACACCCAGAAGCGGAAAATCCCCTACGCGGAT
GCCCCCAGCGTTACGCTTGGCCGTCTGGCAATAGATATCAGCCTACAGAAACAAGGCTACGGGGAGGTTCTCGTAACGCA
CGCGTTGAAGGTTGTTTATCAGGCTTCTCGCGCAGTAGGCATATACGCTTTGTTTGTGGATGCACTAAACCAAAGCGCAA
TGCAGTTCTATCAGAAGTTTGGATTCATCCCTTTGACGGGGGCAAATGCCAACTCGCTTTTCTACCCCACGAAAAGCATT
GAAGAGCTGTTTGAAACAGAGTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT149477 WP_000554758.1 NZ_CP048439:c3646495-3646202 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT149477 NZ_CP064114:c8386-8117 [Enterobacter hormaechei]
ATGTCAGCACTGAAAAAACAGCGCATCGATCTCAGATTAACCGACGACGACAAGAGCATGATCGAAGAAGCTGCGGCAAT
GACCAACCAGACGATTACTCAGTTTATGGTTGCCAGTGCCTCTGAACGTGCTGCGGAAGTGATAGAGCAACATCGCCGCT
TGATTCTCAGTGAAGAGTCCTGGAATCTGGTAATGGATGCCATCAGTAACCCGCCTGCACCGAACGATAGGCTGAAACGA
GCTGCCAAACGTCTACAAAGCATGGAGTAA

Structures

Toxin

• AlphaFold DB: A0A090J8B1

Antitoxin

• AlphaFold DB: S1QAE3