Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1761770..1762272 Replicon chromosome
Accession NZ_CP048439
Organism Escherichia coli strain NBRC 3301

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag GXM24_RS08340 Protein ID WP_000767829.1
Coordinates 1762018..1762272 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag GXM24_RS08335 Protein ID WP_001259255.1
Coordinates 1761770..1762021 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GXM24_RS08310 1756948..1758015 - 1068 WP_000080105.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
GXM24_RS08315 1758015..1759085 - 1071 WP_000108941.1 histidinol-phosphate transaminase -
GXM24_RS08320 1759082..1760386 - 1305 WP_000009594.1 histidinol dehydrogenase -
GXM24_RS08325 1760392..1761291 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
GXM24_RS08330 1761437..1761487 - 51 WP_001364200.1 his operon leader peptide -
GXM24_RS08335 1761770..1762021 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
GXM24_RS08340 1762018..1762272 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
GXM24_RS08345 1762355..1763179 + 825 WP_000754737.1 SDR family oxidoreductase -
GXM24_RS08350 1763225..1764154 + 930 WP_000803351.1 LysR family transcriptional regulator -
GXM24_RS08355 1764369..1764431 + 63 WP_010723108.1 membrane protein YoeI -
GXM24_RS08360 1764421..1765779 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
GXM24_RS08365 1765958..1767016 + 1059 WP_000492339.1 transport protein YeeE -
GXM24_RS08370 1767030..1767257 + 228 WP_000234896.1 sulfurtransferase TusA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T149462 WP_000767829.1 NZ_CP048439:1762018-1762272 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T149462 NZ_CP064108:2593130-2593233 [Klebsiella oxytoca]
GGCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTTTTTTT

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT149462 WP_001259255.1 NZ_CP048439:1761770-1762021 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT149462 NZ_CP064108:c2593240-2593095 [Klebsiella oxytoca]
AAAAGATAAAAAAAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCCTTATAAGAATAGTTTACCGTGTCAGGTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References