Detailed information of TA system TA149448

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 1576211..1576895
• Replicon: chromosome
• Accession: NZ_CP048433
• Organism: Collinsella aerofaciens ATCC 25986 strain JCM 10188

Toxin (Protein)

• Gene name: HigB2
• Uniprot ID: A0A858B5M7
• Locus tag: GXM19_RS07190
• Protein ID: WP_040359593.1
• Coordinates: 1576500..1576895 (-)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GXM19_RS07185
• Protein ID: WP_006235701.1
• Coordinates: 1576211..1576513 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GXM19_RS07155	1571275..1571469	-	195	WP_022094262.1	ATP synthase subunit C	-
GXM19_RS07160	1571594..1572397	-	804	WP_022094263.1	F0F1 ATP synthase subunit A	-
GXM19_RS07165	1572401..1572712	-	312	WP_035138788.1	hypothetical protein	-
GXM19_RS07170	1572993..1573640	-	648	WP_040359658.1	uracil phosphoribosyltransferase	-
GXM19_RS07175	1573763..1575304	-	1542	WP_006235703.1	glycerol kinase GlpK	-
GXM19_RS07180	1575318..1576061	-	744	WP_006235702.1	L-threonylcarbamoyladenylate synthase	-
GXM19_RS07185	1576211..1576513	-	303	WP_006235701.1	XRE family transcriptional regulator	Antitoxin
GXM19_RS07190	1576500..1576895	-	396	WP_040359593.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GXM19_RS07195	1576971..1578227	-	1257	WP_006235699.1	peptide chain release factor N(5)-glutamine methyltransferase	-
GXM19_RS07200	1578823..1579890	-	1068	WP_006235697.1	peptide chain release factor 1	-
GXM19_RS07205	1580136..1581551	-	1416	WP_006235694.1	M20/M25/M40 family metallo-hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (18-118)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-82)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 15133.48 Da
• Isoelectric Point: 10.4003

>T149448 WP_040359593.1 NZ_CP048433:c1576895-1576500 [Collinsella aerofaciens ATCC 25986]
MSNRLHRYRIVLDYIEPWLDEQDEATLKQIYADLLVLEKEGPSLGRPLVDRVKGSKLHHLKELRVTSCGGQVIRILFAFD
PKRQAVLLLAGDKSRAGSSRAKWNGWYAINIPKAEQIYRRHVRRLDRDGTA

Nucleotide

• Length: 396 bp

>T149448 NZ_CP064103:c4083639-4083241 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACAGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11388.92 Da
• Isoelectric Point: 8.5154

>AT149448 WP_006235701.1 NZ_CP048433:c1576513-1576211 [Collinsella aerofaciens ATCC 25986]
MELHEYMESRGITRDSITAEQERTRDRIEAYKLAQIRRLKDLTQASVAQSMGVSQKRVSELERGELGSMRLDTLSRYAES
LGGKLVASIEFPDRTVTLAR

Nucleotide

• Length: 303 bp

>AT149448 NZ_CP064103:c4083935-4083642 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTTGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAACTGGTTAAGGCGCGCTTCCGTCCGAGTGCCGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: A0A858B5M7