Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 4528221..4528867 Replicon chromosome
Accession NZ_CP048430
Organism Klebsiella pneumoniae strain KP18-3-8

Toxin (Protein)


Gene name higB Uniprot ID W8UNE1
Locus tag GZ213_RS23165 Protein ID WP_002920560.1
Coordinates 4528520..4528867 (-) Length 116 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID W8UB68
Locus tag GZ213_RS23160 Protein ID WP_002920557.1
Coordinates 4528221..4528520 (-) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GZ213_RS23140 4524427..4525185 - 759 WP_002920548.1 DeoR/GlpR family transcriptional regulator -
GZ213_RS23145 4525235..4526065 - 831 WP_004151408.1 rhomboid family intramembrane serine protease GlpG -
GZ213_RS23150 4526116..4526445 - 330 WP_002920552.1 thiosulfate sulfurtransferase GlpE -
GZ213_RS23155 4526650..4528158 + 1509 WP_002920554.1 glycerol-3-phosphate dehydrogenase -
GZ213_RS23160 4528221..4528520 - 300 WP_002920557.1 helix-turn-helix transcriptional regulator Antitoxin
GZ213_RS23165 4528520..4528867 - 348 WP_002920560.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
GZ213_RS23170 4529043..4531490 - 2448 WP_002920561.1 glycogen phosphorylase -
GZ213_RS23175 4531508..4532941 - 1434 WP_002920564.1 glycogen synthase GlgA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-107)

Antitoxin

HigA2-like, helix-turn-helix domain

(9-87)


Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 13502.56 Da        Isoelectric Point: 6.2300

>T149431 WP_002920560.1 NZ_CP048430:c4528867-4528520 [Klebsiella pneumoniae]
MWDVETTDTFDAWFELQSRALKEDMLATMLILSEFAPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRACFAFDPLRKAI
VLCVGNKDGMNEKRFYKEMITLADREFSQHLTKER

Download         Length: 348 bp

>T149431 NZ_CP064097:1327724-1328074 [Priestia aryabhattai]
TTGGTAGTCAAACGTGGCGACGTTTATTTTGCTGACCTATCTCCTGTTGTTGGCTCAGAACAAGGAGGCGTTCGCCCTGT
ACTTGTCATTCAAAATGATATAGGCAATCGTTTTAGCCCAACTGTTATTGTAGCTGCGATCACTGCTCAAATTCAAAAGG
CAAAGTTACCTACGCATGTTGAAATTGATGCAAAGCGCTATGGATTTGAGCGAGATTCAGTTATTTTACTAGAACAAATT
CGTACTATTGATAAACAGAGGCTAACAGACAAGATTACCCATTTAGATGATGAAATGATGGACAGAGTGGATGAAGCCTT
GCAAATCAGTGTAGGACTTATCGACTTTTAG

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10800.58 Da        Isoelectric Point: 10.1464

>AT149431 WP_002920557.1 NZ_CP048430:c4528520-4528221 [Klebsiella pneumoniae]
MATLKELMAKQSPDSQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQADNDPRLSTLKRYIAALG
GELSINVTLPTGKKVSFNL

Download         Length: 300 bp

>AT149431 NZ_CP064097:1327438-1327719 [Priestia aryabhattai]
GTGTCTGAAGCGAGCGCAACTACAGAAATCCTAGTAAGATTACCGAAAAATTTAGTATCGGAGCTCGATCTTATGGTTAA
ACAAGAAAATGGCAACCGAAGTGATTTTATTCACCAAGCAACAAAATTGTACCTTCGTGAGCGAAAAAAACGCCATATTC
GCGAATCAATGAGACGTGGATATATGGAGATGGCAAAGATAAACTTAAATATTGCTTCCGAGGCTTTTCTAGCAGAGTAT
GAAGCTGATCATACTGTGGATCGTTTAGTAAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E1CBY5

Antitoxin

Source ID Structure
AlphaFold DB A0A0E1CBF8

References