Detailed information of TA system TA149407

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2425796..2426358
• Replicon: chromosome
• Accession: NZ_CP048416
• Organism: Citrobacter freundii strain CitB

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: GZ999_RS11440
• Protein ID: WP_032936429.1
• Coordinates: 2426080..2426358 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A8B5QC60
• Locus tag: GZ999_RS11435
• Protein ID: WP_003836231.1
• Coordinates: 2425796..2426080 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GZ999_RS11420	2423487..2424371	+	885	WP_003833024.1	formate dehydrogenase N subunit beta	-
GZ999_RS11425	2424364..2425020	+	657	WP_171278982.1	formate dehydrogenase-N subunit gamma	-
GZ999_RS11430	2425150..2425752	+	603	WP_032935729.1	inorganic diphosphatase	-
GZ999_RS11435	2425796..2426080	-	285	WP_003836231.1	HigA family addiction module antidote protein	Antitoxin
GZ999_RS11440	2426080..2426358	-	279	WP_032936429.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GZ999_RS11445	2426579..2428294	-	1716	WP_032935731.1	ATP-binding cassette domain-containing protein	-
GZ999_RS11450	2428604..2430301	-	1698	WP_003020042.1	malate dehydrogenase	-
GZ999_RS11455	2430481..2430621	-	141	WP_003020039.1	stationary-phase-induced ribosome-associated protein	-
GZ999_RS11460	2430849..2431280	+	432	WP_003020036.1	peroxiredoxin OsmC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-83)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10667.30 Da
• Isoelectric Point: 9.8965

>T149407 WP_032936429.1 NZ_CP048416:c2426358-2426080 [Citrobacter freundii]
MIMNFRHKGLRDLFLHGRTSGVMAKHVRRLRHRLAVIDAASKVTDINMPGYKLHPLVGDRDGIWAITVSGNWRITFEFVN
GDAYILDYEDYH

Nucleotide

• Length: 279 bp

>T149407 NZ_CP064094:121194-121940 [Bacillus subtilis]
ATGGTTTTATTCTTTCAGATCATGGTCTGGTGCATCGTGGCCGGACTGGGGTTATACGTGTATGCCACGTGGCGTTTCGA
AGCGAAGGTCAAAGAAAAAATGTCCGCCATTCGGAAAACTTGGTATTTGCTGTTTGTTCTGGGCGCTATGGTATACTGGA
CATATGAGCCCACTTCCCTATTTACCCACTGGGAACGGTATCTCATTGTCGCAGTCAGTTTTGCTTTGATTGATGCTTTT
ATCTTCTTAAGTGCATATGTCAAAAAACTGGCCGGCAGCGAGCTTGAAACAGACACAAGAGAAATTCTTGAAGAAAACAA
CGAAATGCTCCACATGTATCTCAATCGGCTGAAAACATACCAATACCTATTGAAAAACGAACCGATCCATGTTTATTATG
GAAGTATAGATGCTTATGCTGAAGGTATTGATAAGCTGCTGAAAACCTATGCTGATAAAATGAACTTAACGGCTTCTCTT
TGCCACTATTCGACACAGGCTGATAAAGACCGGTTAACCGAGCATATGGATGATCCGGCAGATGTACAAACACGGCTCGA
TCGAAAGGATGTTTATTACGACCAATACGGAAAAGTGGTTCTCATCCCTTTTACCATCGAGACACAGAACTATGTCATCA
AGCTGACGTCTGACAGCATTGTCACGGAATTTGATTATTTGCTATTTACGTCATTAACGAGCATATATGATTTGGTGCTG
CCAATTGAGGAGGAAGGTGAAGGATAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10358.10 Da
• Isoelectric Point: 8.8094

>AT149407 WP_003836231.1 NZ_CP048416:c2426080-2425796 [Citrobacter freundii]
MKMANHPRPGDIIQEALEELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPEMWLNLQTTWSLAEAQ
KSVDISRLRKLAVQ

Nucleotide

• Length: 285 bp

>AT149407 NZ_CP064094:121940-122110 [Bacillus subtilis]
ATGGAACGTGCGTTTCAAAACAGATGCGAGCCCAGAGCGGCGAAGCCGTTTAAAATCCTGAAAAAACGTTCAACCACCAG
TGTCGCAAGCTATCAAGTCAGTCCGCATACAGCAAGAATCTTCAAAGAAAACGAACGGCTGATTGACGAGTATAAACGAA
AAAAAGCATGA

Structures

Antitoxin

• AlphaFold DB: A0A8B5QC60