Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 2049381..2050207 Replicon chromosome
Accession NZ_CP048411
Organism Pseudomonas sp. MTM4

Toxin (Protein)


Gene name yhaV Uniprot ID L0GFH6
Locus tag GYM54_RS09345 Protein ID WP_015275816.1
Coordinates 2049381..2049884 (-) Length 168 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID L0GI82
Locus tag GYM54_RS09350 Protein ID WP_015275815.1
Coordinates 2049881..2050207 (-) Length 109 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GYM54_RS09320 (GYM54_09305) 2044932..2045330 + 399 WP_003291554.1 VOC family protein -
GYM54_RS09325 (GYM54_09310) 2045682..2046851 + 1170 WP_231752243.1 DUF3422 family protein -
GYM54_RS09330 (GYM54_09315) 2046955..2047149 - 195 Protein_1833 transposase -
GYM54_RS09335 (GYM54_09320) 2047354..2048367 - 1014 WP_015275817.1 helix-turn-helix domain-containing protein -
GYM54_RS09340 (GYM54_09325) 2048434..2048736 + 303 WP_007182542.1 antibiotic biosynthesis monooxygenase -
GYM54_RS09345 (GYM54_09330) 2049381..2049884 - 504 WP_015275816.1 type II toxin-antitoxin system YhaV family toxin Toxin
GYM54_RS09350 (GYM54_09335) 2049881..2050207 - 327 WP_015275815.1 type II toxin-antitoxin system PrlF family antitoxin Antitoxin
GYM54_RS09355 (GYM54_09340) 2050309..2050752 - 444 Protein_1838 inositol monophosphatase -
GYM54_RS09360 (GYM54_09345) 2050760..2051071 - 312 Protein_1839 transposase -
GYM54_RS09365 (GYM54_09350) 2051221..2052210 - 990 WP_041756905.1 zinc-dependent alcohol dehydrogenase family protein -
GYM54_RS09370 (GYM54_09355) 2052351..2053247 + 897 WP_079329350.1 LysR family transcriptional regulator -
GYM54_RS09375 (GYM54_09360) 2053316..2053522 + 207 WP_197445814.1 hypothetical protein -
GYM54_RS09380 (GYM54_09365) 2054230..2055138 - 909 WP_197445815.1 LysR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 2050832..2051071 239


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(16-152)

Antitoxin

PrlF antitoxin

(8-106)


Sequences

Toxin        


Download         Length: 168 a.a.        Molecular weight: 19223.69 Da        Isoelectric Point: 9.5348

>T149388 WP_015275816.1 NZ_CP048411:c2049884-2049381 [Pseudomonas sp. MTM4]
MSQDQGKPLVAHGWTVFAHPLFLDQLETLIQQVELSKQKDPVGYRKNNAAKRLAAITKLAFDVIPQDPSKPEYRQGGTFG
EEHKHWFRAKFFQQYRLFFRYHGPSKVIILAWVNDEDTKRAYESSDDAYKVFRKMLASGHPPDSWDKLLNEATQENARLN
SAATSVN

Download         Length: 504 bp

>T149388 NZ_CP064057:3339357-3339614 [Streptomyces sp. XC 2026]
GTGAGGAACGTCGACTTAGACCCGGCCGCCTGGGACGACTTTCTGTTCTGGCTCGCGTCGGACAGGAAGACAGCCCGGCG
GATCACTCGTCTCATCGCCGAGGTCCAACGCACCCCGTTCGAGGGCATCGGCAAACCGGAGCCGCTGAAGGGTGACCTCA
CGGGTTACTGGTCGCGGCGGATCAATGACGAACACCGGCTCGTCTGCCGCACCGGCGACAAGGAAATGAAGATCATCAAG
GCCCGCTACCACTACTGA

Antitoxin


Download         Length: 109 a.a.        Molecular weight: 12019.35 Da        Isoelectric Point: 4.4621

>AT149388 WP_015275815.1 NZ_CP048411:c2050207-2049881 [Pseudomonas sp. MTM4]
MARALEVESTLTDRYQTTVPETVRRALQLNKRDKIHYSIRPSGEVVLSRAEPTEDDPAIGQFLSFLAHDIAANPQHLRAI
DSDLVARIHSLVDSNDIDLDAPLSADDE

Download         Length: 327 bp

>AT149388 NZ_CP064057:3339085-3339360 [Streptomyces sp. XC 2026]
ATGAGTATCAGTGCCAGTGAAGCCAGAAAGAGCCTGTTTCCGCTCATCGAACGCGTCAACACCGACCACGACCCGGTGCG
GATCACGTCCAAGGCCGGGGACGCCGTCCTGATGTCCGCCGATGACTACGACTCCTGGCAGGAGACCATCTACTTTCTGC
GTTCCCCCGCCAATGCCCAGCGGCTCATGGAGGCGGTCGCCCGGGACAAAGCGGGCGAGGCCGGAGCCGCCAGGACGCTC
GACGAGCTCCGGAACATGGCTGGGGCTGAGGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB L0GFH6

Antitoxin

Source ID Structure
AlphaFold DB A0A5R9QF20

References