Detailed information of TA system TA149385

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 5755479..5756059
• Replicon: chromosome
• Accession: NZ_CP048408
• Organism: Pseudomonas fluorescens strain DR397

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: GZH78_RS25830
• Protein ID: WP_085747693.1
• Coordinates: 5755479..5755757 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GZH78_RS25835
• Protein ID: WP_045121630.1
• Coordinates: 5755772..5756059 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GZH78_RS25810	5751061..5751228	-	168	WP_085668053.1	DUF2474 domain-containing protein	-
GZH78_RS25815	5751286..5752293	-	1008	WP_039771762.1	cytochrome d ubiquinol oxidase subunit II	-
GZH78_RS25820	5752297..5753733	-	1437	WP_039771763.1	cytochrome ubiquinol oxidase subunit I	-
GZH78_RS25825	5754134..5755372	+	1239	WP_103385470.1	MFS transporter	-
GZH78_RS25830	5755479..5755757	+	279	WP_085747693.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GZH78_RS25835	5755772..5756059	+	288	WP_045121630.1	HigA family addiction module antidote protein	Antitoxin
GZH78_RS25840	5756065..5756616	-	552	WP_163976526.1	DJ-1/PfpI family protein	-
GZH78_RS25845	5756684..5757133	-	450	WP_163976528.1	YolA family protein	-
GZH78_RS25850	5757354..5758649	+	1296	WP_007950999.1	NCS2 family permease	-
GZH78_RS25855	5758646..5759725	+	1080	WP_039771766.1	tRNA (uridine(54)-C5)-methyltransferase TrmA	-
GZH78_RS25860	5759780..5760226	-	447	WP_039771767.1	hypothetical protein	-
GZH78_RS25865	5760223..5760651	-	429	WP_074691513.1	DUF4160 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (3-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (20-75)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10673.22 Da
• Isoelectric Point: 8.9401

>T149385 WP_085747693.1 NZ_CP048408:5755479-5755757 [Pseudomonas fluorescens]
MIKSFQHKGLRGFYETGSTRGIRADHAKRLSRMLQFMDRAAAPGDLDLPGWRLHPLKGELCEYWSLSVSGNWRVIFRFAG
SDIELIDYLDYH

Nucleotide

• Length: 279 bp

>T149385 NZ_CP064055:c3912982-3912476 [Morganella morganii subsp. morganii]
TTGAACTTAACGGCACCGGAACCCTTGCAGCAGCATCATGATTTTACCGTTTTTCAGTCAGGTGAGGAGTCCCTTAACCA
TTGGCTGAAAACCCGCGCATTGCAAAATCAGCAATCCGGCGCTTCACGGACATTTGTCGTCTGCCACCATAACCGGATAA
TGGCCTATTATGCATTATCGACAGGCGTTATTACCTGTTCACAGGCGGCGGGTCGTTTCAGACGTAATATGCCCCCGGAA
ATTCCGGTTATCCTGCCGGGACGTCTTGCTGTTGACGAAAGCGTAAAAGGCAGAGGGATCGGACGGGGTCTGATAAAAGA
TGCCGCACTGCGGGTCTTGCAGGCGGCCGGGATTGTGGGTATCCGCGGTATTGTTGTCCGTGCTCTGTCTGATAATGCCA
GACGTTTTTATGAGCATACCGGTTTTATGCCGTCACCGGCTGACCCGATGCTGCTGATGATTACATTACGGGATCTGCAG
CTGGCAACCGGCATCTATTCTGAATAG

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10542.17 Da
• Isoelectric Point: 6.9219

>AT149385 WP_045121630.1 NZ_CP048408:5755772-5756059 [Pseudomonas fluorescens]
MPMHNPPHPGETLLMDVLPELGISVTELARHLGFARPHLSRVLHGHAPISPDLAVRLERAGIGKARMWLGVQTDYDLWQA
EHREQPAIQPIAAHA

Nucleotide

• Length: 288 bp

>AT149385 NZ_CP064055:c3913293-3912985 [Morganella morganii subsp. morganii]
ATGACAAAACACACAGATGAGCAGCATCCTGTGTCCGGCGTGAATATCACCCGTTCAACACTGAATTTACGCATCAAACC
GGAAGATAAAATTCTGATTGATCGCGCAGCGCATGCTGCCGGTAAAAACAGAACCGAGTTCGTACTGGAAGCTGCGCGGC
GTGCCGCCGAAGAAACACTGGCAGATTTACGGGTTATTGATGTCAGTACCGATGTCTATCAGCAGTTTATTACCAGACTG
GATATGGCACCACAGTCCGGTGAGGCGCTGAAGAAGACCATGCTGGCGAAGGCGCCGTGGGAGAAGTAA