Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 726642..727336 Replicon chromosome
Accession NZ_CP048408
Organism Pseudomonas fluorescens strain DR397

Toxin (Protein)


Gene name higB Uniprot ID J2Q035
Locus tag GZH78_RS03300 Protein ID WP_007952335.1
Coordinates 726642..726938 (+) Length 99 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A2N8H690
Locus tag GZH78_RS03305 Protein ID WP_039766491.1
Coordinates 726935..727336 (+) Length 134 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GZH78_RS03265 722307..722852 - 546 WP_007952346.1 hypothetical protein -
GZH78_RS03270 722849..723103 - 255 WP_007947135.1 acyl carrier protein -
GZH78_RS03275 723113..723373 - 261 WP_085701087.1 acyl carrier protein -
GZH78_RS03280 723354..724163 - 810 WP_163973893.1 1-acyl-sn-glycerol-3-phosphate acyltransferase -
GZH78_RS03285 724139..724864 - 726 WP_064592952.1 beta-ketoacyl synthase chain length factor -
GZH78_RS03290 725234..726004 + 771 WP_007952339.1 ParA family protein -
GZH78_RS03295 726012..726446 - 435 WP_064592954.1 thioredoxin TrxC -
GZH78_RS03300 726642..726938 + 297 WP_007952335.1 type II toxin-antitoxin system HigB family toxin Toxin
GZH78_RS03305 726935..727336 + 402 WP_039766491.1 transcriptional regulator Antitoxin
GZH78_RS03310 727469..728932 - 1464 WP_163973894.1 YdiU family protein -
GZH78_RS03315 728981..732334 - 3354 WP_039766495.1 mechanosensitive channel MscK -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-90)

Antitoxin

Cro/C1-type helix-turn-helix domain

(78-132)


Sequences

Toxin        


Download         Length: 99 a.a.        Molecular weight: 11297.14 Da        Isoelectric Point: 10.6259

>T149381 WP_007952335.1 NZ_CP048408:726642-726938 [Pseudomonas fluorescens]
MHVITGKRIREAKAKWPNAATALDHWLEVAKGSKSPDFAALKALFPAVDKVGDFHVFDIGGNKLRLIAYVRYRPQKLYIR
HVLDHREYDRGKWKEAKA

Download         Length: 297 bp

>T149381 NZ_CP064054:c861701-861498 [Morganella morganii subsp. morganii]
ATGACTAAACTGGACTATCTCATGCGCTTACGTAAGTGCACTTCTATTGAAACACTGGAACGTGTCATTGAGAAAAACAA
ATATGAACTGACTGATGATGAGCTGGAGGTTTTTTATTCAGCAGCCGATCATCGTCTGGCCGAATTAACCATGAATAAAC
TGTATGACAAAATCCCGGCATCTGTCTGGAAATTTGTCCGTTAA

Antitoxin


Download         Length: 134 a.a.        Molecular weight: 15294.51 Da        Isoelectric Point: 4.8251

>AT149381 WP_039766491.1 NZ_CP048408:726935-727336 [Pseudomonas fluorescens]
MSVLIKKAAEHWEFVSPLLRKPKSEDDYDRLVQALDELLEMTGDDESHPLMSLVDIIGDWIEEWDRVHHPTPKPPGFEVL
GYLMREHGLTQSDLPGVGTQSVVSEILSGKRQLNLRQVRWLAEHFKLPVDVFI

Download         Length: 402 bp

>AT149381 NZ_CP064054:c862124-861765 [Morganella morganii subsp. morganii]
ATGGACGAGTATTCTCCACGAAATTACGATATTGCGGTACTGAAGTATCTGTGTGATGAACTTTGCCGTGAAGGCACACA
AACCCTCATCAGAAGCAATAATTATTGGGTTAATGATTTAGCCTCAGATTCAAACTTAAAACTTAACGAATTATTAGGTT
ACATTGCTGACGTGGCCTGGAGTTTTAAGATAAAACATACACAAAATAAGGATCTCATCTCATTTGTCGATGAATACATC
GATGAAACTTACGCGCTGTTTGGTCAGATGGAAGTTTCGTTTAATGACGTTGAGGAATGGAAGAAGATGGCATCACTGGT
AAGCAGCATGCTGACCAGCGAGGGGCATCTTGTCCACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB J2Q035

Antitoxin

Source ID Structure
AlphaFold DB A0A2N8H690

References