Detailed information of TA system TA149379

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 538855..539459
• Replicon: chromosome
• Accession: NZ_CP048408
• Organism: Pseudomonas fluorescens strain DR397

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: GZH78_RS02395
• Protein ID: WP_163973850.1
• Coordinates: 539145..539459 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GZH78_RS02390
• Protein ID: WP_115076185.1
• Coordinates: 538855..539142 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GZH78_RS02375	534697..536238	+	1542	WP_007952682.1	phosphoenolpyruvate carboxykinase	-
GZH78_RS02380	536287..536928	-	642	WP_163973848.1	hypothetical protein	-
GZH78_RS02385	536925..537797	-	873	WP_163973849.1	hypothetical protein	-
GZH78_RS02390	538855..539142	-	288	WP_115076185.1	helix-turn-helix domain-containing protein	Antitoxin
GZH78_RS02395	539145..539459	-	315	WP_163973850.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GZH78_RS02400	539571..539870	-	300	WP_115076187.1	hypothetical protein	-
GZH78_RS02405	540421..541548	-	1128	WP_074689457.1	hypothetical protein	-
GZH78_RS02410	541651..541932	-	282	WP_039764597.1	hypothetical protein	-
GZH78_RS02415	542237..542740	+	504	WP_074689459.1	polysaccharide deacetylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (42-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (35-86)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11847.76 Da
• Isoelectric Point: 10.3534

>T149379 WP_163973850.1 NZ_CP048408:c539459-539145 [Pseudomonas fluorescens]
MIFVETRIFTRRVKELLDDDTYAAFQKQLVVSPSIGDVIEGTGGIRKTRIAAKGHGKRGGARVIYYHFVSASQIGLLMIY
PKNEQHDLSSDERKALKLLIEKWR

Nucleotide

• Length: 315 bp

>T149379 NZ_CP064051:48318-48668 [Enterococcus faecium]
ATGGTGAGAAAACCTAGACAAGGCGATATTCTATTATTGAATACAGCACCTCGATCTGGACACGAACAGACAGGTAAAAG
ACCTTATATTGTTTTAAGTCATGATATTATTGCGGATTATAGTAACGTTGTTACAGTTGCTCCAATTTCTAGTACAACTA
GAAACTATCCGCTATATGTAAATATAAACTCAGAATATGAAATGAAGACAACAGGAAAAGTATTATTAGATCAACTAACG
ACAATTGATTATGAAGCCAGAGAGTGTATTTTTTTGGAAAAAGCGCATGATAGTTTAGTTGAGGAGTTATTGATGAAAGT
TAGAACAGTTTTTCAAAAAGTAAGCAAATAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10713.31 Da
• Isoelectric Point: 6.7193

>AT149379 WP_115076185.1 NZ_CP048408:c539142-538855 [Pseudomonas fluorescens]
MSKFFDELMESVQEMDEIVRGERTPSREFHVDALQVKEIRKATGLTQAKFAALIDVQLGTLRNWEQGRREPTGPAKALLK
AIHNDPVHVLNALAC

Nucleotide

• Length: 288 bp

>AT149379 NZ_CP064051:48061-48324 [Enterococcus faecium]
ATGGAAATGTTACCTATAAAAAAATGGGGAAATAGTAATGGCCTTAGATTGCCAAAGTATATTATGGAATATCTTGGAAT
TCATACAGAAGACAAAGTGAAGATAATACAAGAAGAAGTAAACGGTCAGAAGCGGCTAATTATCGAAGCGGCTAATATAG
AAAATGAATTAACAATTGAACAGTTATTTGAAAATTACACTGATGAAAGAAATCATGTGACGATTCAAAATTTAGGTGAA
GCAGTAGGTAATGAAAAATGGTGA