Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 3910673..3911270 Replicon chromosome
Accession NZ_CP048389
Organism Klebsiella pneumoniae strain S01

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag GZH33_RS18970 Protein ID WP_004142563.1
Coordinates 3910953..3911270 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag GZH33_RS18965 Protein ID WP_004142561.1
Coordinates 3910673..3910960 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GZH33_RS18935 (3906753) 3906753..3907001 + 249 WP_020325243.1 DUF1158 domain-containing protein -
GZH33_RS18940 (3907019) 3907019..3907360 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
GZH33_RS18945 (3907391) 3907391..3908506 - 1116 WP_020325248.1 MBL fold metallo-hydrolase -
GZH33_RS18950 (3908686) 3908686..3909267 + 582 WP_020325240.1 TetR/AcrR family transcriptional regulator -
GZH33_RS18955 (3909267) 3909267..3909635 + 369 WP_020325252.1 MmcQ/YjbR family DNA-binding protein -
GZH33_RS18960 (3909755) 3909755..3910408 + 654 WP_004178896.1 oxygen-insensitive NAD(P)H nitroreductase -
GZH33_RS18965 (3910673) 3910673..3910960 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
GZH33_RS18970 (3910953) 3910953..3911270 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
GZH33_RS18975 (3911455) 3911455..3912498 - 1044 WP_020325241.1 DUF2157 domain-containing protein -
GZH33_RS18980 (3913168) 3913168..3914034 - 867 WP_004151823.1 helix-turn-helix transcriptional regulator -
GZH33_RS18985 (3914143) 3914143..3915570 + 1428 WP_009308097.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T149359 WP_004142563.1 NZ_CP048389:c3911270-3910953 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T149359 NZ_CP064035:c1944225-1943971 [Vibrio parahaemolyticus]
ATGAGAATCGTATCTTTTACTGAAGCTAGAAATGGTCTCAAAGCTGTTTTAGACGGTGTAGTTAATGATGCTGATACAAC
AGTTATTACACGCCGTGATTCTGAGGATGCAGTGGTTATGTCTTTAGACTACTACAATAGCCTTATGGAGACTGTTCATT
TACTACGCTCTCCTCAAAACGTTGAACACCTAAACCGTTCGATAGCACAGTACCGCGCTGGTAAAACAACAGCACGAGAG
TTAATTGATGAGTAG

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT149359 WP_004142561.1 NZ_CP048389:c3910960-3910673 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT149359 NZ_CP064035:c1943978-1943709 [Vibrio parahaemolyticus]
ATGAGTAGTAGTCAACGTTTATTATCGTGGACTGATGATGCTTGGGATGACTACCTGTATTGGCAAACTCAAGACAAGAA
AACACTCAAGCGCATCAATAAACTCATCAATGATGTTAAGCGCTCTCCATTTGAGGGCATTGGTAAACCAGAGCCGTTAA
AAGAGAACTTATCTGGTTTTTGGTCTCGTCGTATTGATGATACTAATAGGCTTGTTTATGCAGTCGATGATCAAGCGATA
ACGATAATTTCATGTCGTTACCACTACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References