Detailed information of TA system TA149346

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4651135..4651889
• Replicon: chromosome
• Accession: NZ_CP048388
• Organism: Citrobacter portucalensis strain PNUCL1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A5B0T5Y1
• Locus tag: GZH81_RS21830
• Protein ID: WP_048215202.1
• Coordinates: 4651135..4651446 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GZH81_RS21835
• Protein ID: WP_048215203.1
• Coordinates: 4651443..4651889 (+)
• Length: 149 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GZH81_RS21800 (4646797)	4646797..4647699	+	903	WP_212024551.1	formate dehydrogenase O subunit beta	-
GZH81_RS21805 (4647696)	4647696..4648331	+	636	WP_003028686.1	formate dehydrogenase cytochrome b556 subunit	-
GZH81_RS21810 (4648328)	4648328..4649257	+	930	WP_003028685.1	formate dehydrogenase accessory protein FdhE	-
GZH81_RS21815 (4649301)	4649301..4649588	-	288	WP_038635893.1	NadS family protein	-
GZH81_RS21820 (4649589)	4649589..4649900	-	312	WP_048227551.1	hypothetical protein	-
GZH81_RS21825 (4650121)	4650121..4651050	+	930	WP_111466282.1	alpha/beta hydrolase	-
GZH81_RS21830 (4651135)	4651135..4651446	+	312	WP_048215202.1	type II toxin-antitoxin system HigB family toxin	Toxin
GZH81_RS21835 (4651443)	4651443..4651889	+	447	WP_048215203.1	helix-turn-helix domain-containing protein	Antitoxin
GZH81_RS21840 (4651907)	4651907..4652848	-	942	WP_003825286.1	fatty acid biosynthesis protein FabY	-
GZH81_RS21845 (4652893)	4652893..4653330	-	438	WP_003825289.1	D-aminoacyl-tRNA deacylase	-
GZH81_RS21850 (4653327)	4653327..4654199	-	873	WP_008786998.1	virulence factor BrkB family protein	-
GZH81_RS21855 (4654193)	4654193..4654792	-	600	WP_103015344.1	glucose-1-phosphatase	-
GZH81_RS21860 (4654883)	4654883..4655773	-	891	WP_212024553.1	aldose 1-epimerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (93-146)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12345.28 Da
• Isoelectric Point: 9.5337

>T149346 WP_048215202.1 NZ_CP048388:4651135-4651446 [Citrobacter portucalensis]
VHVISRKPFNEAMLMYPNHQLALADLLNVLEKKTFIQPEEMKQYIPSLDNFKYRDKWWVIDVSGNSLRLISYIDFRSHKI
FVKHIVSHAEYDKLTAYYRGNKE

Nucleotide

• Length: 312 bp

>T149346 NZ_CP064005:4103605-4103757 [Xanthomonas translucens pv. translucens]
GTGCTAGTCAGGATCCACCATGGCCCCGGATATCGGGTGTATTTCCAGCGGCGCGGCGACACGATCATCGTCTTGCTCTG
CGGTGGCGACAAAAGCACGCAGGCAAAGGACATCAAGACAGCCAAACGGCTTGCCGACGAATGGAGCGAATGA

Antitoxin

Protein

• Length: 149 a.a.
• Molecular weight: 16660.97 Da
• Isoelectric Point: 6.7580

>AT149346 WP_048215203.1 NZ_CP048388:4651443-4651889 [Citrobacter portucalensis]
MRTHRHMDAASAKKIVDTFSDAVKTIPLLGEDRNDTEYRRALALVEYLVDHDDLENPLFELLCARISKYEKHAPEFKALN
QHLEKTPSGVSVLRTLMDQYSLKAADLANELGSKSNVSNILNGRRALTVSHIKALAERFNLPADAFIE

Nucleotide

• Length: 447 bp

>AT149346 NZ_CP064005:4103760-4104053 [Xanthomonas translucens pv. translucens]
ATGACCGAAAACCTGACCACCTACGATCCTGCCGAGGATCTGAACTCCGACCAGGCGATTGCCGATTTCATGGCGGGCGC
CTTTGCGACCAACGACACCGGCTTCATCGCCCATGCCCTCGGCATCGTCGCCCGTGCCAAGGGCATGACCCAGATCGCAC
GACAAACCGGCCTGTCCCGGGAGCAGCTCTACCGCTCCTTCAGCGAGGATGGAAATCCGACGCTGCGCACCACCCTGGCC
GTCATGAAGGCGCTCGGGATCGAACTGACGGCCAAAGCCGCCGTCACGCATTGA

Structures

Toxin

• AlphaFold DB: A0A5B0T5Y1