Detailed information of TA system TA149337

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2429046..2429608
• Replicon: chromosome
• Accession: NZ_CP048388
• Organism: Citrobacter portucalensis strain PNUCL1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: GZH81_RS11390
• Protein ID: WP_048236430.1
• Coordinates: 2429330..2429608 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A6H3AUF8
• Locus tag: GZH81_RS11385
• Protein ID: WP_003833018.1
• Coordinates: 2429046..2429330 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GZH81_RS11370 (2426741)	2426741..2427625	+	885	WP_003833024.1	formate dehydrogenase N subunit beta	-
GZH81_RS11375 (2427618)	2427618..2428274	+	657	WP_003020054.1	formate dehydrogenase-N subunit gamma	-
GZH81_RS11380 (2428404)	2428404..2429006	+	603	WP_003833020.1	inorganic diphosphatase	-
GZH81_RS11385 (2429046)	2429046..2429330	-	285	WP_003833018.1	HigA family addiction module antitoxin	Antitoxin
GZH81_RS11390 (2429330)	2429330..2429608	-	279	WP_048236430.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GZH81_RS11395 (2429829)	2429829..2431544	-	1716	WP_212023628.1	ABC-F family ATP-binding cassette domain-containing protein	-
GZH81_RS11400 (2431854)	2431854..2433551	-	1698	WP_003833011.1	malate dehydrogenase	-
GZH81_RS11405 (2433731)	2433731..2433871	-	141	WP_003833009.1	stationary-phase-induced ribosome-associated protein	-
GZH81_RS11410 (2434099)	2434099..2434530	+	432	WP_003833007.1	peroxiredoxin OsmC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-83)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10598.26 Da
• Isoelectric Point: 9.5471

>T149337 WP_048236430.1 NZ_CP048388:c2429608-2429330 [Citrobacter portucalensis]
MIMNFRHKGLRDLFLHGRTSGVMAKHVRRLRHRLAVIDAASKVTEINMPGYKLHPLVGERDGIWAISVSGNCRITFEFVN
GDAYILDYEDYH

Nucleotide

• Length: 279 bp

>T149337 NZ_CP064004:2580233-2580490 [Xanthomonas translucens pv. translucens]
GTGACGCTTCAGTTCTCGGACAATGCCTGGGAGGACGACCTCTACTGGCAGCAGACCGACAAGAAGATGCTCAAGCGCAT
CAACGACCTGATCAAAGCCATTCAACGCGATCCCTTCCAGGGCGTCGGCAAGCCAGAACGGCTACGCCATGCCTTGGCCG
GCTATTGGTCGCGGCGCATCAACGACGAACACCGCATCGTCTACAAGGTCGAGGCGGGCGTCCTACTGATCGCGCAGGTG
CGGTATCACTACGCGTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10371.10 Da
• Isoelectric Point: 8.8094

>AT149337 WP_003833018.1 NZ_CP048388:c2429330-2429046 [Citrobacter portucalensis]
MKMANHPRPGDIIQEALEELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPEMWLNLQNTWSLAEAQ
KSVDISRLRKLAVQ

Nucleotide

• Length: 285 bp

>AT149337 NZ_CP064004:2579985-2580236 [Xanthomonas translucens pv. translucens]
ATGGACACCATCACCTATAGCGCTGCACGCGCCGCCCTTGCGGACACCATGGAGCGTGTCGTCAACAATCACGAGCCGGT
GATCATCACCCGCAGCCGCGAGCAATCCGTGGTCATGCTGTCGCTGGAGGACTACAAGGCGATGGAGGAAACCGCCTACC
TGCTGCGCAGCCCGAAGAGCGCGCAGCGCCTGCTGGAATCCATCGCCCAGCTAGAGGCCGGTCGCGGCAAGGCACGGGAC
CTGGCCGAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A6H3AUF8