Detailed information of TA system TA149309

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1966423..1967093
• Replicon: chromosome
• Accession: NZ_CP048382
• Organism: Citrobacter freundii strain 62

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A6N6JXY6
• Locus tag: GZS04_RS09395
• Protein ID: WP_046671374.1
• Coordinates: 1966423..1966725 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GZS04_RS09400
• Protein ID: WP_046671375.1
• Coordinates: 1966752..1967093 (+)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GZS04_RS09380	1961966..1963489	-	1524	WP_003844755.1	sugar ABC transporter ATP-binding protein	-
GZS04_RS09385	1963604..1965868	+	2265	WP_044702173.1	hybrid sensor histidine kinase/response regulator	-
GZS04_RS09390	1965938..1966267	+	330	WP_003031807.1	hypothetical protein	-
GZS04_RS09395	1966423..1966725	+	303	WP_046671374.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GZS04_RS09400	1966752..1967093	+	342	WP_046671375.1	HigA family addiction module antidote protein	Antitoxin
GZS04_RS09405	1967146..1967904	-	759	WP_046671376.1	phosphonate metabolism protein PhnP	-
GZS04_RS09410	1967913..1968347	-	435	WP_003031809.1	aminoalkylphosphonate N-acetyltransferase	-
GZS04_RS09415	1968334..1968888	-	555	WP_003844764.1	ribose 1,5-bisphosphokinase	-
GZS04_RS09420	1968891..1970027	-	1137	WP_003031811.1	alpha-D-ribose 1-methylphosphonate 5-triphosphate diphosphatase	-
GZS04_RS09425	1970024..1970704	-	681	WP_046671387.1	phosphonate C-P lyase system protein PhnL	-
GZS04_RS09430	1970794..1971552	-	759	WP_003844769.1	phosphonate C-P lyase system protein PhnK	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (9-98)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-70)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11819.43 Da
• Isoelectric Point: 9.9581

>T149309 WP_046671374.1 NZ_CP048382:1966423-1966725 [Citrobacter freundii]
MSKSLNIRSFRDTWLEDFFERATSHRKIPADIHTALARKLDIINAAVSHRDLRSPPGNRYEELTGKLQEYSSIRVNKQYR
LIFKWVNGKAEDVYLDPHIY

Nucleotide

• Length: 303 bp

>T149309 NZ_CP063996:3179355-3179612 [Xanthomonas translucens pv. undulosa]
GTGACGCTTCAGTTCTCGGACAATGCCTGGGAGGACTACCTCTACTGGCAGCAGACCGACAAGAAGATGCTCAAGCGCAT
CAACGACCTGATCAAAGCCATTCAACGCGATCCCTTCCAGGGCGTCGGCAAGCCAGAACCGCTACGCCATGCCTTGGCCG
GCTATTGGTCGCGGCGCATCAACGACGAACATCGCATCGTGTACAAGGTCGAGGCGGGCGTCCTGCTGATCGCGCAGGTG
CGCTACCACTACGTGTAG

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 13017.84 Da
• Isoelectric Point: 7.2040

>AT149309 WP_046671375.1 NZ_CP048382:1966752-1967093 [Citrobacter freundii]
MKQATRKPTTVGDILQYEYLEPLGLKINDLAEILHVHRNTVSALVNNNRKLTTDMAFRLAKAFDTSVDFWRNLQAAVDLW
EVENDMRAQEELNRIVSVKDFIAQRNSESKKVA

Nucleotide

• Length: 342 bp

>AT149309 NZ_CP063996:3179107-3179358 [Xanthomonas translucens pv. undulosa]
ATGGACACCATCACCTATAGCGCTGCACGCGCCGCCCTTGCGGACACCATGGAGCGCGTCGTCAACAACCACGAGCCGGT
GATCATCACCCGCAGCCGCGAGCAATCCGTGGTCATGCTGTCGCTTGAAGACTACAAGGCGATGGAGGAAACCGCCTACC
TGCTCCGCAGTCCGAAGAGCGCGCAGCGCCTGCTGGAATCCATCGCCCAGCTCGAGGCCGGTCGCGGCAAGGCACGGGAC
CTGGCCGAGTGA

Structures

Toxin

• AlphaFold DB: A0A6N6JXY6