Detailed information of TA system TA149298

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 46084..46323
• Replicon: plasmid p38_A-OXA140
• Accession: NZ_CP048377
• Organism: Escherichia coli strain 38

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: A0A762TWR7
• Locus tag: GZS06_RS22965
• Protein ID: WP_023144756.1
• Coordinates: 46084..46218 (-)
• Length: 45 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	46263..46323 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GZS06_RS22935	41343..42040	-	698	Protein_52	IS6 family transposase	-
GZS06_RS22940	42067..42650	-	584	Protein_53	incFII family plasmid replication initiator RepA	-
GZS06_RS22945	42643..42717	-	75	WP_031943482.1	RepA leader peptide Tap	-
GZS06_RS23350	42714..42848	-	135	Protein_55	protein CopA/IncA	-
GZS06_RS22950	43079..44613	+	1535	Protein_56	IS21 family transposase	-
GZS06_RS22955	44628..45373	+	746	Protein_57	IS21-like element ISEc12 family helper ATPase IstB	-
GZS06_RS22960	45533..45787	-	255	WP_000083850.1	replication regulatory protein RepA	-
GZS06_RS22965	46084..46218	-	135	WP_023144756.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	46263..46323	+	61	NuclAT_0	-	Antitoxin
-	46263..46323	+	61	NuclAT_0	-	Antitoxin
-	46263..46323	+	61	NuclAT_0	-	Antitoxin
-	46263..46323	+	61	NuclAT_0	-	Antitoxin
GZS06_RS22970	46290..46576	-	287	Protein_60	DUF2726 domain-containing protein	-
GZS06_RS22975	47089..47301	-	213	WP_013023861.1	hypothetical protein	-
GZS06_RS22980	47432..47992	-	561	WP_000139341.1	fertility inhibition protein FinO	-
GZS06_RS22985	48047..48619	-	573	Protein_63	type-F conjugative transfer system pilin acetylase TraX	-
GZS06_RS22990	48658..49716	-	1059	Protein_64	DUF3560 domain-containing protein	-
GZS06_RS22995	49768..49998	-	231	WP_000218642.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	tet(B) / sul1 / qacE / aadA5 / blaNDM-5 / aadA2 / dfrA12 / blaTEM-1B / aac(6')-Ib-cr / blaOXA-1 / blaCTX-M-15	-	1..86996	86996

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-44)

Sequences

Toxin

Protein

• Length: 45 a.a.
• Molecular weight: 4896.90 Da
• Isoelectric Point: 7.7209

>T149298 WP_023144756.1 NZ_CP048377:c46218-46084 [Escherichia coli]
MTKYTLIGLLAVCATVLCFSLIFREQLCELNIHRGNTVVQVTLA

Nucleotide

• Length: 135 bp

>T149298 NZ_CP063992:c4168526-4168424 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin

Nucleotide

• Length: 61 bp

>AT149298 NZ_CP048377:46263-46323 [Escherichia coli]
TAAAGTGCATCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A762TWR7

Antitoxin