Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1086278..1087005 Replicon chromosome
Accession NZ_CP048376
Organism Escherichia coli strain 38

Toxin (Protein)


Gene name higB Uniprot ID J7Q991
Locus tag GZS06_RS05245 Protein ID WP_000547564.1
Coordinates 1086278..1086589 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag GZS06_RS05250 Protein ID WP_000126294.1
Coordinates 1086586..1087005 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GZS06_RS05215 1081420..1083129 + 1710 WP_001288134.1 formate hydrogenlyase subunit HycE -
GZS06_RS05220 1083139..1083681 + 543 WP_000493792.1 formate hydrogenlyase subunit HycF -
GZS06_RS05225 1083681..1084448 + 768 WP_000067399.1 formate hydrogenlyase subunit HycG -
GZS06_RS05230 1084445..1084855 + 411 WP_001291921.1 formate hydrogenlyase assembly protein HycH -
GZS06_RS05235 1084848..1085318 + 471 WP_060621146.1 hydrogenase maturation peptidase HycI -
GZS06_RS05240 1085343..1086104 + 762 WP_001026446.1 hypothetical protein -
GZS06_RS05245 1086278..1086589 + 312 WP_000547564.1 type II toxin-antitoxin system HigB family toxin Toxin
GZS06_RS05250 1086586..1087005 + 420 WP_000126294.1 helix-turn-helix domain-containing protein Antitoxin
GZS06_RS05255 1087119..1088543 - 1425 WP_000110363.1 6-phospho-beta-glucosidase AscB -
GZS06_RS05260 1088552..1090009 - 1458 WP_001107861.1 PTS cellobiose/arbutin/salicin transporter subunit IIBC -
GZS06_RS05265 1090269..1091279 + 1011 WP_001392554.1 DNA-binding transcriptional regulator AscG -
GZS06_RS05270 1091428..1091955 + 528 WP_001078777.1 electron transport protein HydN -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12426.13 Da        Isoelectric Point: 9.7248

>T149282 WP_000547564.1 NZ_CP048376:1086278-1086589 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

>T149282 NZ_CP063983:4269322-4269531 [Escherichia coli]
ATGCAGAACACATGTAGAGTGCCTCTTACTGACCGAAAGGTCAAGGAGAAGAGAGCAATGAAGCAGCATAAGGCGATGAT
TGTCGCCCTGATCGTCATCTGTATCACCGCCGTAGTGGCGGCGCTGGTAACGAGAAAAGACCTCTGTGAGGTTCACATCC
GAACTGGCCAGACGGAGGTTGCTGTTTTCACGGCTTACGAATCCGAGTAA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15416.42 Da        Isoelectric Point: 4.4596

>AT149282 WP_000126294.1 NZ_CP048376:1086586-1087005 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Download         Length: 420 bp

>AT149282 NZ_CP063983:c4269330-4269273 [Escherichia coli]
GTTCTGCATATAGGGGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A0FJG5

Antitoxin

Source ID Structure

References