Detailed information of TA system TA149273

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 66205..66469
• Replicon: plasmid pC-F-163_B
• Accession: NZ_CP048373
• Organism: Escherichia coli strain 163

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Z417
• Locus tag: GZS08_RS23845
• Protein ID: WP_001387489.1
• Coordinates: 66205..66357 (-)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	66409..66469 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GZS08_RS23810	61770..61886	+	117	Protein_68	IncI1-type conjugal transfer lipoprotein TraH	-
GZS08_RS23815	61885..63639	+	1755	Protein_69	DotA/TraY family protein	-
GZS08_RS23820	63710..64372	+	663	WP_000644796.1	plasmid IncI1-type surface exclusion protein ExcA	-
GZS08_RS23825	64444..64653	-	210	WP_000062603.1	HEAT repeat domain-containing protein	-
GZS08_RS23830	65045..65221	+	177	WP_001054897.1	hypothetical protein	-
GZS08_RS23835	65286..65582	-	297	WP_011264046.1	hypothetical protein	-
GZS08_RS23840	65882..66133	+	252	WP_001291964.1	hypothetical protein	-
GZS08_RS23845	66205..66357	-	153	WP_001387489.1	Hok/Gef family protein	Toxin
-	66409..66469	+	61	NuclAT_0	-	Antitoxin
-	66409..66469	+	61	NuclAT_0	-	Antitoxin
-	66409..66469	+	61	NuclAT_0	-	Antitoxin
-	66409..66469	+	61	NuclAT_0	-	Antitoxin
GZS08_RS23850	66672..67019	+	348	Protein_76	protein finQ	-
GZS08_RS23855	67205..68362	+	1158	Protein_77	IS1380-like element ISEc9 family transposase	-
GZS08_RS23860	68418..69115	+	698	Protein_78	IS1 family transposase	-
GZS08_RS23865	69129..69434	-	306	Protein_79	transcription termination factor NusG	-
GZS08_RS23870	69876..70163	-	288	WP_000074855.1	conjugal transfer protein TraA	-
GZS08_RS23875	70081..70344	-	264	WP_032180564.1	ash family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCMY-42	-	1..71270	71270

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5761.10 Da
• Isoelectric Point: 8.7948

>T149273 WP_001387489.1 NZ_CP048373:c66357-66205 [Escherichia coli]
MPQRTFLMMLIVVCVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T149273 NZ_CP063983:2220361-2220463 [Escherichia coli]
GCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Nucleotide

• Length: 61 bp

>AT149273 NZ_CP048373:66409-66469 [Escherichia coli]
GAGGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: U9Z417

Antitoxin