Detailed information of TA system TA149268

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3751496..3752190
• Replicon: chromosome
• Accession: NZ_CP048371
• Organism: Escherichia coli strain 163

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q47157
• Locus tag: GZS08_RS17890
• Protein ID: WP_001263489.1
• Coordinates: 3751496..3751894 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: GZS08_RS17895
• Protein ID: WP_000554758.1
• Coordinates: 3751897..3752190 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	3747084..3747164	-	81	NuclAT_11	-	-
-	3747084..3747164	-	81	NuclAT_11	-	-
-	3747084..3747164	-	81	NuclAT_11	-	-
-	3747084..3747164	-	81	NuclAT_11	-	-
GZS08_RS17865	3747760..3748218	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
GZS08_RS17870	3748479..3749936	+	1458	WP_001292994.1	cytosol nonspecific dipeptidase	-
GZS08_RS17875	3749993..3750514	-	522	Protein_3497	peptide chain release factor H	-
GZS08_RS17880	3750510..3750716	-	207	Protein_3498	RtcB family protein	-
GZS08_RS17885	3751034..3751486	-	453	WP_001059892.1	GNAT family N-acetyltransferase	-
GZS08_RS17890	3751496..3751894	-	399	WP_001263489.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
GZS08_RS17895	3751897..3752190	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
GZS08_RS17900	3752242..3753297	-	1056	WP_001226164.1	DNA polymerase IV	-
GZS08_RS17905	3753368..3754153	-	786	WP_000207552.1	putative lateral flagellar export/assembly protein LafU	-
GZS08_RS17910	3754125..3755837	+	1713	Protein_3504	flagellar biosynthesis protein FlhA	-
GZS08_RS17915	3756061..3756558	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3681701..3757360	75659

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15487.84 Da
• Isoelectric Point: 7.4215

>T149268 WP_001263489.1 NZ_CP048371:c3751894-3751496 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T149268 NZ_CP063983:997803-998210 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGAAAACGGCA
ACATTTATGGCTGGCAGCTGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTACAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT149268 WP_000554758.1 NZ_CP048371:c3752190-3751897 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT149268 NZ_CP063983:997556-997822 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTTTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A090J8B1

Antitoxin

• AlphaFold DB: S1QAE3