Detailed information of TA system TA149243

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 88898..89319
• Replicon: plasmid pC-F-164_A-OXA140
• Accession: NZ_CP048368
• Organism: Escherichia coli strain 164

Toxin (Protein)

• Gene name: hok
• Uniprot ID: B1VC78
• Locus tag: GZS07_RS23970
• Protein ID: WP_001302184.1
• Coordinates: 89161..89319 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	88898..89096 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GZS07_RS23935	84601..85995	+	1395	WP_001037799.1	carbohydrate porin	-
GZS07_RS23940	86241..86345	-	105	Protein_93	thiosulfate reductase cytochrome B subunit	-
GZS07_RS23945	86402..87088	+	687	Protein_94	IS1 family transposase	-
GZS07_RS23950	87104..87727	+	624	WP_000912556.1	potassium channel family protein	-
GZS07_RS23955	87812..88063	+	252	Protein_96	iron-containing alcohol dehydrogenase	-
GZS07_RS23960	88352..88669	-	318	Protein_97	IS110 family transposase	-
GZS07_RS23965	88824..88929	+	106	Protein_98	plasmid SOS inhibition protein A	-
-	88898..89096	+	199	NuclAT_0	-	Antitoxin
-	88898..89096	+	199	NuclAT_0	-	Antitoxin
-	88898..89096	+	199	NuclAT_0	-	Antitoxin
-	88898..89096	+	199	NuclAT_0	-	Antitoxin
GZS07_RS23970	89161..89319	+	159	WP_001302184.1	type I toxin-antitoxin system Hok family toxin	Toxin
GZS07_RS24365	89987..90193	+	207	WP_000275854.1	hypothetical protein	-
GZS07_RS23980	90218..90505	+	288	WP_000107242.1	hypothetical protein	-
GZS07_RS23985	90624..91445	+	822	WP_001234465.1	DUF945 domain-containing protein	-
GZS07_RS23990	91741..92313	-	573	WP_000949660.1	transglycosylase SLT domain-containing protein	-
GZS07_RS23995	92683..93066	+	384	WP_001063020.1	relaxosome protein TraM	-
GZS07_RS24000	93257..93904	+	648	WP_000332520.1	conjugal transfer transcriptional regulator TraJ	-
GZS07_RS24005	94040..94255	+	216	WP_001352842.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aac(6')-Ib-cr / blaOXA-1 / blaCTX-M-15 / dfrA12 / aadA2 / qacE / sul1 / blaNDM-5 / rmtB / blaTEM-1B / mph(A)	-	1..132482	132482
	-	flank	IS/Tn	-	-	86741..87088	347

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6108.35 Da
• Isoelectric Point: 9.1977

>T149243 WP_001302184.1 NZ_CP048368:89161-89319 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T149243 NZ_CP063954:5266547-5267464 [Klebsiella variicola]
ATGGATATACAGGCGCCCTATTATCGGCAGGTTGCTCTGTTAATGCAGGTTTTACCCTATGTTGCCGTCGAACGGGAATT
TGCGCTGAAAGGTGGCACGGCAATCAATCTCTTTATCCGTGATTTCCCACGATTATCGGTGGATATCGACCTCGCCTGGG
TGCCCCTGGAAAGCAGAGCGATCGCTTTACCTCATATACGGGATGCGCTTGCACGTATTGCCGCTAATCTGCAACAACAG
GCAGGTATAAACGCTGTATTACAAGCTAATCGTTCCGATGAGATGCGTGTCGTTGTTACTACCGATAGCGCACAAATAAA
GATAGAGGTCTCACCGGTCGCACGGGGAACATTATATCCACCACAGGAGCGAGAGGTGGTGGGGGCGGTAGAGGACGTTT
TTGGCTATGCCAGCCTTCCGGTGGTTTCGTTGCCCGATCTCTACGGTGGAAAATTATGCGCGGCGCTGGATCGCCAGCAT
CCCAGAGATTTTTATGATGTGAAATTACTTCTTGATGCGCAGGAGCTTAACCGTCCCATTTTCAATGGGTTTATCGCGTA
TTTACTTAGCCATAATCGGCCTCTGGCTGAAGTGCTTAGTCCTCGCTGGAAAGATATTGCTGAACCCTTTTACCGTGAAT
TCAGCGGGATGACATTTGAGACGATAGCCCTGGAGGAACTGACAGCAGTCCCCTACAGGATGATTGATGCGTTAAAATCC
TGTTTCACGCAACAGGATATCGATTTTTTGCTGTCATTTAAACGAGGCGAACCAGACTGGCGTTTAGCGCCGGAAACACG
TATTCAGGATTTGCCTGCCGTGCAGTGGAAATTGCGCAATATTCATCAGATGCCAGCGATAAAACGCGCTGAGTCATTAG
ATAAACTGGAAAAGGTGTTGGCCGAGTGGCGCAGTTAG

Antitoxin

Nucleotide

• Length: 199 bp

>AT149243 NZ_CP048368:88898-89096 [Escherichia coli]
TCACACAGATTACCCGTAAACAGTCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Antitoxin