Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3830223..3830917 Replicon chromosome
Accession NZ_CP048367
Organism Escherichia coli strain 164

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag GZS07_RS18465 Protein ID WP_001263489.1
Coordinates 3830223..3830621 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag GZS07_RS18470 Protein ID WP_000554758.1
Coordinates 3830624..3830917 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- 3825811..3825891 - 81 NuclAT_13 - -
- 3825811..3825891 - 81 NuclAT_13 - -
- 3825811..3825891 - 81 NuclAT_13 - -
- 3825811..3825891 - 81 NuclAT_13 - -
GZS07_RS18440 3826487..3826945 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
GZS07_RS18445 3827206..3828663 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
GZS07_RS18450 3828720..3829241 - 522 Protein_3606 peptide chain release factor H -
GZS07_RS18455 3829237..3829443 - 207 Protein_3607 RtcB family protein -
GZS07_RS18460 3829761..3830213 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
GZS07_RS18465 3830223..3830621 - 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
GZS07_RS18470 3830624..3830917 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
GZS07_RS18475 3830969..3832024 - 1056 WP_001226164.1 DNA polymerase IV -
GZS07_RS18480 3832095..3832880 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
GZS07_RS18485 3832825..3834564 + 1740 WP_001334802.1 flagellar biosynthesis protein FlhA -
GZS07_RS18490 3834788..3835285 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T149236 WP_001263489.1 NZ_CP048367:c3830621-3830223 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T149236 NZ_CP063954:c2962982-2962880 [Klebsiella variicola]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT149236 WP_000554758.1 NZ_CP048367:c3830917-3830624 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT149236 NZ_CP063954:2962874-2963018 [Klebsiella variicola]
ACCGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGT
TGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References