Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1930899..1931401 Replicon chromosome
Accession NZ_CP048367
Organism Escherichia coli strain 164

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag GZS07_RS09185 Protein ID WP_000767829.1
Coordinates 1931147..1931401 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag GZS07_RS09180 Protein ID WP_001259255.1
Coordinates 1930899..1931150 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GZS07_RS09155 1926077..1927144 - 1068 WP_000080136.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
GZS07_RS09160 1927144..1928214 - 1071 WP_000108941.1 histidinol-phosphate transaminase -
GZS07_RS09165 1928217..1929515 - 1299 WP_087760826.1 histidinol dehydrogenase -
GZS07_RS09170 1929521..1930420 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
GZS07_RS09175 1930566..1930616 - 51 WP_001364200.1 his operon leader peptide -
GZS07_RS09180 1930899..1931150 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
GZS07_RS09185 1931147..1931401 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
GZS07_RS09190 1931484..1932308 + 825 WP_000754762.1 SDR family oxidoreductase -
GZS07_RS09195 1932354..1933283 + 930 WP_087760827.1 LysR family transcriptional regulator -
GZS07_RS09200 1933498..1933560 + 63 WP_010723108.1 membrane protein YoeI -
GZS07_RS09205 1933550..1934908 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
GZS07_RS09210 1935087..1936145 + 1059 WP_000492324.1 transport protein YeeE -
GZS07_RS09215 1936159..1936386 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T149222 WP_000767829.1 NZ_CP048367:1931147-1931401 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T149222 NZ_CP063953:c67872-67555 [Klebsiella variicola]
ATGTTCAGAATAGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAAATAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT149222 WP_001259255.1 NZ_CP048367:1930899-1931150 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT149222 NZ_CP063953:c67562-67275 [Klebsiella variicola]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTAGCCAGCCTGAGGTTCAGGCTGCGTTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTGACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACGGTCTCCAGAATGGAAGCCAATGTCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGTGGAATAAAGCACCCACAAATTTTACTTTACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References