Detailed information of TA system TA149210

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 41839..42103
• Replicon: plasmid p53_B
• Accession: NZ_CP048361
• Organism: Escherichia coli strain 53

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: E6BRV3
• Locus tag: GZS09_RS24575
• Protein ID: WP_001303307.1
• Coordinates: 41951..42103 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	41839..41901 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GZS09_RS24560	37078..39369	-	2292	WP_001289271.1	hypothetical protein	-
GZS09_RS24565	39362..40432	-	1071	WP_000151576.1	IncI1-type conjugal transfer protein TrbB	-
GZS09_RS24570	40451..41659	-	1209	WP_000121273.1	IncI1-type conjugal transfer protein TrbA	-
-	41839..41901	-	63	NuclAT_0	-	Antitoxin
-	41839..41901	-	63	NuclAT_0	-	Antitoxin
-	41839..41901	-	63	NuclAT_0	-	Antitoxin
-	41839..41901	-	63	NuclAT_0	-	Antitoxin
GZS09_RS24575	41951..42103	+	153	WP_001303307.1	Hok/Gef family protein	Toxin
GZS09_RS24580	42175..42426	-	252	WP_001291965.1	hypothetical protein	-
-	42813..42864	-	52	NuclAT_1	-	-
-	42813..42864	-	52	NuclAT_1	-	-
-	42813..42864	-	52	NuclAT_1	-	-
-	42813..42864	-	52	NuclAT_1	-	-
GZS09_RS25715	43350..43526	-	177	WP_001054900.1	hypothetical protein	-
GZS09_RS24585	43735..43944	-	210	WP_001140545.1	hemolysin expression modulator Hha	-
GZS09_RS24590	44042..44656	-	615	WP_000578649.1	plasmid IncI1-type surface exclusion protein ExcA	-
GZS09_RS24595	44732..46900	-	2169	WP_000698360.1	IncI1-type conjugal transfer membrane protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..88513	88513

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5817.21 Da
• Isoelectric Point: 8.7948

>T149210 WP_001303307.1 NZ_CP048361:41951-42103 [Escherichia coli]
MPQRTFLMMLIVICVTILCFVWMVRDSLCVLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T149210 NZ_CP063945:c3717548-3717264 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCTGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Nucleotide

• Length: 63 bp

>AT149210 NZ_CP048361:c41901-41839 [Escherichia coli]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: A0A634Q5L1

Antitoxin