Detailed information of TA system TA149203

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 79955..80209
• Replicon: plasmid p53_A
• Accession: NZ_CP048360
• Organism: Escherichia coli strain 53

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: GZS09_RS23860
• Protein ID: WP_015387399.1
• Coordinates: 80003..80209 (+)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	79955..80016 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GZS09_RS23825	75607..75819	+	213	WP_005012601.1	hypothetical protein	-
GZS09_RS25695	76120..76209	-	90	Protein_91	IS1 family transposase	-
GZS09_RS23835	76264..76941	+	678	WP_001339397.1	IS66 family insertion sequence hypothetical protein	-
GZS09_RS23840	76941..77288	+	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-
GZS09_RS23845	77308..78879	+	1572	WP_023149734.1	IS66 family transposase	-
GZS09_RS23850	78917..79533	-	617	Protein_95	IS1 family transposase	-
GZS09_RS23855	79634..79816	+	183	WP_000968309.1	hypothetical protein	-
-	79955..80016	-	62	NuclAT_1	-	Antitoxin
-	79955..80016	-	62	NuclAT_1	-	Antitoxin
-	79955..80016	-	62	NuclAT_1	-	Antitoxin
-	79955..80016	-	62	NuclAT_1	-	Antitoxin
GZS09_RS23860	80003..80209	+	207	WP_015387399.1	type I toxin-antitoxin system Hok family toxin	Toxin
GZS09_RS23865	80493..80750	+	258	WP_000083833.1	replication regulatory protein RepA	-
GZS09_RS23870	80986..81060	+	75	WP_032336874.1	RepA leader peptide Tap	-
GZS09_RS23875	81053..81499	+	447	Protein_100	incFII family plasmid replication initiator RepA	-
GZS09_RS23880	81445..81927	-	483	Protein_101	VENN motif pre-toxin domain-containing protein	-
GZS09_RS23885	82820..84040	+	1221	WP_000410951.1	arginine deiminase	-
GZS09_RS23890	84051..84962	+	912	WP_000440183.1	carbamate kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-27 / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / mph(A) / sul1 / blaDHA-1 / qnrB4 / sitABCD	-	1..144585	144585

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7720.23 Da
• Isoelectric Point: 9.3127

>T149203 WP_015387399.1 NZ_CP048360:80003-80209 [Escherichia coli]
MKYLNTTDCSLFLAGRSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T149203 NZ_CP063943:5141276-5141623 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATACCGGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTCCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTTTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGCGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT149203 NZ_CP048360:c80016-79955 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin