Detailed information of TA system TA149199

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 39853..40123
• Replicon: plasmid p53_A
• Accession: NZ_CP048360
• Organism: Escherichia coli strain 53

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: GZS09_RS23625
• Protein ID: WP_001312861.1
• Coordinates: 39965..40123 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	39853..39916 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GZS09_RS23580	35964..36529	+	566	Protein_42	class I SAM-dependent methyltransferase	-
GZS09_RS23585	36555..36773	+	219	WP_001496291.1	hypothetical protein	-
GZS09_RS25690	37183..37389	+	207	WP_000275856.1	hypothetical protein	-
GZS09_RS23595	37415..37954	+	540	WP_000290840.1	single-stranded DNA-binding protein	-
GZS09_RS23600	38022..38255	+	234	WP_000005990.1	DUF905 family protein	-
GZS09_RS23605	38283..38480	+	198	Protein_47	hypothetical protein	-
GZS09_RS23610	38535..38969	+	435	WP_000845937.1	conjugation system SOS inhibitor PsiB	-
GZS09_RS23615	38966..39685	+	720	WP_001276238.1	plasmid SOS inhibition protein A	-
GZS09_RS23620	39697..39885	-	189	WP_001299721.1	hypothetical protein	-
-	39697..39921	+	225	NuclAT_0	-	-
-	39697..39921	+	225	NuclAT_0	-	-
-	39697..39921	+	225	NuclAT_0	-	-
-	39697..39921	+	225	NuclAT_0	-	-
-	39853..39916	-	64	-	-	Antitoxin
GZS09_RS23625	39965..40123	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
GZS09_RS23635	41044..41331	+	288	WP_000107535.1	hypothetical protein	-
GZS09_RS23640	41450..42271	+	822	WP_001234426.1	DUF945 domain-containing protein	-
GZS09_RS23645	42568..43170	-	603	WP_013362798.1	transglycosylase SLT domain-containing protein	-
GZS09_RS23650	43491..43874	+	384	WP_001151524.1	relaxosome protein TraM	-
GZS09_RS23655	44061..44750	+	690	WP_000283385.1	conjugal transfer transcriptional regulator TraJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-27 / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / mph(A) / sul1 / blaDHA-1 / qnrB4 / sitABCD	-	1..144585	144585

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T149199 WP_001312861.1 NZ_CP048360:39965-40123 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T149199 NZ_CP063943:c4236773-4236489 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCTGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCTATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Nucleotide

• Length: 64 bp

>AT149199 NZ_CP048360:c39916-39853 [Escherichia coli]
GACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCTT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin