Detailed information of TA system TA149198

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4668562..4669164
• Replicon: chromosome
• Accession: NZ_CP048359
• Organism: Escherichia coli strain 53

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: GZS09_RS22325
• Protein ID: WP_000897305.1
• Coordinates: 4668853..4669164 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GZS09_RS22320
• Protein ID: WP_000356397.1
• Coordinates: 4668562..4668852 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GZS09_RS22295	4664488..4665390	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
GZS09_RS22300	4665387..4666022	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
GZS09_RS22305	4666019..4666948	+	930	WP_085668558.1	formate dehydrogenase accessory protein FdhE	-
GZS09_RS22310	4667278..4667520	-	243	WP_001086388.1	hypothetical protein	-
GZS09_RS22315	4667739..4667957	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
GZS09_RS22320	4668562..4668852	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
GZS09_RS22325	4668853..4669164	-	312	WP_000897305.1	hypothetical protein	Toxin
GZS09_RS22330	4669393..4670301	+	909	WP_001162704.1	alpha/beta hydrolase	-
GZS09_RS22335	4670365..4671306	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
GZS09_RS22340	4671351..4671788	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
GZS09_RS22345	4671785..4672657	-	873	WP_085668556.1	virulence factor BrkB family protein	-
GZS09_RS22350	4672651..4673250	-	600	WP_001295269.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T149198 WP_000897305.1 NZ_CP048359:c4669164-4668853 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T149198 NZ_CP063943:c4163277-4162963 [Klebsiella pneumoniae]
ATTTCAACTGTACCGGCCAAGGTGCCGGTTTCGTCACGTCCGTCGCCCGTGCAGGTCTGGCAGCAACTTTTAACGTATCT
GCTGGATCACCATTACGGCCTGACGCTTAACGACACACCATTCCATGACGACGGGGCCATCGAGGAGCATATCGATGCAG
GAATCACGCTCGCCGATGCTGTTAATTTTCTGGTGGAACGTTATGAACTGGTACGCATCGACCGCAAAGGGTTTAGCTGG
CAGGAACAGACTCCGTTCCTGACCGCGACTGATATTCTCCGAGCCAGGCGAGCTACCGGATTAACAAATACTTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT149198 WP_000356397.1 NZ_CP048359:c4668852-4668562 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT149198 NZ_CP063943:c4163643-4163320 [Klebsiella pneumoniae]
ATGTCAATCACACCACCTCATGAATGGGGTCTACAAAGCGATATCACCCCTCGATTTGCCGCAAGATTAGTTCAGGAAGG
CAACCGGCTGTATTACCTGGCTGACAGGGCTGGATTGACGGGGAGTTTTAGTCCAATGCACCTGCAGAAATTAGATCTGG
CTTTCCCGCTGTTTGTCGAACAACTGGAAGATATGCTGTGTGCTGGCGAACTAAATCCACATCAGCAACGTCAGGTGAAA
ATTCAACTTGCGAGTTTAACCTGTATAGCTGATACCCTTGGTAGCTGCGGTTACGTTTACATTTCTATTTATCCCACACC
GTAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7