Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1798925..1799427 Replicon chromosome
Accession NZ_CP048359
Organism Escherichia coli strain 53

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag GZS09_RS08530 Protein ID WP_000767829.1
Coordinates 1799173..1799427 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag GZS09_RS08525 Protein ID WP_001259255.1
Coordinates 1798925..1799176 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GZS09_RS08500 1794104..1795171 - 1068 WP_001594218.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
GZS09_RS08505 1795171..1796241 - 1071 WP_001594217.1 histidinol-phosphate transaminase -
GZS09_RS08510 1796238..1797542 - 1305 WP_001594215.1 histidinol dehydrogenase -
GZS09_RS08515 1797548..1798447 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
GZS09_RS08520 1798593..1798643 - 51 WP_001364200.1 his operon leader peptide -
GZS09_RS08525 1798925..1799176 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
GZS09_RS08530 1799173..1799427 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
GZS09_RS08535 1799510..1800334 + 825 WP_000754763.1 SDR family oxidoreductase -
GZS09_RS08540 1800380..1801309 + 930 WP_000803351.1 LysR family transcriptional regulator -
GZS09_RS08545 1801524..1801586 + 63 WP_010723108.1 membrane protein YoeI -
GZS09_RS08550 1801576..1802934 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
GZS09_RS08555 1803113..1804171 + 1059 WP_023568741.1 transport protein YeeE -
GZS09_RS08560 1804185..1804412 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T149178 WP_000767829.1 NZ_CP048359:1799173-1799427 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T149178 NZ_CP063939:2573001-2573219 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT149178 WP_001259255.1 NZ_CP048359:1798925-1799176 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT149178 NZ_CP063939:2572600-2572974 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References