Detailed information of TA system TA149164

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 58600..58839
• Replicon: plasmid p124_A
• Accession: NZ_CP048345
• Organism: Escherichia coli strain 124

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: A0A762TWR7
• Locus tag: GZS11_RS23055
• Protein ID: WP_023144756.1
• Coordinates: 58600..58734 (-)
• Length: 45 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	58779..58839 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GZS11_RS23025	54313..55169	-	857	Protein_66	incFII family plasmid replication initiator RepA	-
GZS11_RS23035	55162..55236	-	75	WP_031943482.1	RepA leader peptide Tap	-
GZS11_RS23950	55233..55366	-	134	Protein_68	protein CopA/IncA	-
GZS11_RS23040	55597..57133	+	1537	Protein_69	IS21 family transposase	-
GZS11_RS23045	57148..57892	+	745	Protein_70	IS21-like element ISEc12 family helper ATPase IstB	-
GZS11_RS23050	58052..58306	-	255	WP_162676766.1	replication regulatory protein RepA	-
GZS11_RS23055	58600..58734	-	135	WP_023144756.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	58779..58839	+	61	NuclAT_0	-	Antitoxin
-	58779..58839	+	61	NuclAT_0	-	Antitoxin
-	58779..58839	+	61	NuclAT_0	-	Antitoxin
-	58779..58839	+	61	NuclAT_0	-	Antitoxin
GZS11_RS23060	58806..59092	-	287	Protein_73	DUF2726 domain-containing protein	-
GZS11_RS23065	59549..59815	-	267	WP_162676767.1	hypothetical protein	-
GZS11_RS23070	59943..60503	-	561	WP_000139341.1	fertility inhibition protein FinO	-
GZS11_RS23075	60624..62422	-	1799	Protein_76	AAA family ATPase	-
GZS11_RS23080	62392..63474	-	1083	WP_060621339.1	DUF3560 domain-containing protein	-
GZS11_RS23085	63526..63756	-	231	WP_000218642.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	tet(B) / sul1 / qacE / aadA5 / blaTEM-1B / aac(3)-IId / mph(A) / aac(6')-Ib-cr / blaOXA-1 / blaCTX-M-15 / sul2 / aph(3'')-Ib / aph(6)-Id	-	1..97111	97111

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-44)

Sequences

Toxin

Protein

• Length: 45 a.a.
• Molecular weight: 4896.90 Da
• Isoelectric Point: 7.7209

>T149164 WP_023144756.1 NZ_CP048345:c58734-58600 [Escherichia coli]
MTKYTLIGLLAVCATVLCFSLIFREQLCELNIHRGNTVVQVTLA

Nucleotide

• Length: 135 bp

>T149164 NZ_CP063936:c415492-415390 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin

Nucleotide

• Length: 61 bp

>AT149164 NZ_CP048345:58779-58839 [Escherichia coli]
TAAAGTGCATCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A762TWR7

Antitoxin