Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1832791..1833293 Replicon chromosome
Accession NZ_CP048344
Organism Escherichia coli strain 124

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag GZS11_RS08690 Protein ID WP_000767829.1
Coordinates 1833039..1833293 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag GZS11_RS08685 Protein ID WP_001259255.1
Coordinates 1832791..1833042 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GZS11_RS08660 1827968..1829035 - 1068 WP_000080060.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
GZS11_RS08665 1829035..1830105 - 1071 WP_000108941.1 histidinol-phosphate transaminase -
GZS11_RS08670 1830102..1831406 - 1305 WP_001317939.1 histidinol dehydrogenase -
GZS11_RS08675 1831412..1832311 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
GZS11_RS08680 1832457..1832507 - 51 WP_001364200.1 his operon leader peptide -
GZS11_RS08685 1832791..1833042 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
GZS11_RS08690 1833039..1833293 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
GZS11_RS08695 1833376..1834200 + 825 WP_000754758.1 SDR family oxidoreductase -
GZS11_RS08700 1834246..1835175 + 930 WP_000803351.1 LysR family transcriptional regulator -
GZS11_RS08705 1835390..1835452 + 63 WP_010723108.1 membrane protein YoeI -
GZS11_RS08710 1835442..1836800 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
GZS11_RS08715 1836979..1838037 + 1059 WP_000492346.1 transport protein YeeE -
GZS11_RS08720 1838051..1838278 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T149150 WP_000767829.1 NZ_CP048344:1833039-1833293 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T149150 NZ_CP063934:c275374-275060 [Klebsiella pneumoniae]
ATTTCAACTGTACCGGCCAAGGTGCCGGTTTCGTCACGTCCGTCGCCCGTGCAGGTCTGGCAGCAACTTTTAACGTATCT
GCTGGATCACCATTACGGCCTGACGCTTAACGACACACCATTCCATGACGACGGGGCCATCGAGGAGCATATCGATGCAG
GAATCACGCTCGCCGATGCTGTTAATTTTCTGGTGGAACGTTATGAACTGGTACGCATCGACCGCAAAGGGTTTAGCTGG
CAGGAACAGACTCCGTTCCTGACCGCGACTGATATTCTCCGAGCCAGGCGAGCTACCGGATTAACAAATACTTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT149150 WP_001259255.1 NZ_CP048344:1832791-1833042 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT149150 NZ_CP063934:c275740-275417 [Klebsiella pneumoniae]
ATGTCAATCACACCACCTCATGAATGGGGTCTACAAAGCGATATCACCCCTCGATTTGCCGCAAGATTAGTTCAGGAAGG
CAACCGGCTGTATTACCTGGCTGACAGGGCTGGATTGACGGGGAGTTTTAGTCCAATGCACCTGCAGAAATTAGATCTGG
CTTTCCCGCTGTTTGTCGAACAACTGGAAGATATGCTGTGTGCTGGCGAACTAAATCCACATCAGCAACGTCAGGTGAAA
ATTCAACTTGCGAGTTTAACCTGTATAGCTGATACCCTTGGTAGCTGCGGTTACGTTTACATTTCTATTTATCCCACACC
GTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References