Detailed information of TA system TA149148

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1087365..1088092
• Replicon: chromosome
• Accession: NZ_CP048344
• Organism: Escherichia coli strain 124

Toxin (Protein)

• Gene name: higB
• Uniprot ID: J7Q991
• Locus tag: GZS11_RS05240
• Protein ID: WP_000547564.1
• Coordinates: 1087365..1087676 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GZS11_RS05245
• Protein ID: WP_000126294.1
• Coordinates: 1087673..1088092 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GZS11_RS05210	1082507..1084216	+	1710	WP_001288134.1	formate hydrogenlyase subunit HycE	-
GZS11_RS05215	1084226..1084768	+	543	WP_000493792.1	formate hydrogenlyase subunit HycF	-
GZS11_RS05220	1084768..1085535	+	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
GZS11_RS05225	1085532..1085942	+	411	WP_001291921.1	formate hydrogenlyase assembly protein HycH	-
GZS11_RS05230	1085935..1086405	+	471	WP_060621146.1	hydrogenase maturation peptidase HycI	-
GZS11_RS05235	1086430..1087191	+	762	WP_124039055.1	hypothetical protein	-
GZS11_RS05240	1087365..1087676	+	312	WP_000547564.1	type II toxin-antitoxin system HigB family toxin	Toxin
GZS11_RS05245	1087673..1088092	+	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
GZS11_RS05250	1088206..1089630	-	1425	WP_000110363.1	6-phospho-beta-glucosidase AscB	-
GZS11_RS05255	1089639..1091096	-	1458	WP_001107861.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
GZS11_RS05260	1091356..1092366	+	1011	WP_001392554.1	DNA-binding transcriptional regulator AscG	-
GZS11_RS05265	1092515..1093042	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12426.13 Da
• Isoelectric Point: 9.7248

>T149148 WP_000547564.1 NZ_CP048344:1087365-1087676 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T149148 NZ_CP063933:4659120-4659437 [Klebsiella variicola]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAAATAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTATGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT149148 WP_000126294.1 NZ_CP048344:1087673-1088092 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT149148 NZ_CP063933:4659430-4659717 [Klebsiella variicola]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTAGCCAGCCTGAGGTTCAGGCTGCGTTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTGACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCAACGGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGTGGAATAAAGCACCCACAAATTTTACTTTACTAA

Structures

Toxin

• AlphaFold DB: A0A0A0FJG5