Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 632087..632886 Replicon chromosome
Accession NZ_CP048344
Organism Escherichia coli strain 124

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag GZS11_RS03045 Protein ID WP_000347251.1
Coordinates 632087..632551 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag GZS11_RS03050 Protein ID WP_001307405.1
Coordinates 632551..632886 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GZS11_RS03015 627088..627522 - 435 WP_000948824.1 PTS sugar transporter subunit IIA -
GZS11_RS03020 627540..628418 - 879 WP_001295548.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
GZS11_RS03025 628408..629187 - 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
GZS11_RS03030 629198..629671 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
GZS11_RS03035 629694..630974 - 1281 WP_000681903.1 tagatose-bisphosphate aldolase subunit KbaZ -
GZS11_RS03040 631223..632032 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
GZS11_RS03045 632087..632551 - 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
GZS11_RS03050 632551..632886 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
GZS11_RS03055 633035..634606 - 1572 WP_001273761.1 galactarate dehydratase -
GZS11_RS03060 634981..636315 + 1335 WP_124039064.1 galactarate/glucarate/glycerate transporter GarP -
GZS11_RS03065 636331..637101 + 771 WP_001058214.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T149144 WP_000347251.1 NZ_CP048344:c632551-632087 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T149144 NZ_CP063933:c2936791-2936423 [Klebsiella variicola]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCGGC
AGCGGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGCGGATGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT149144 WP_001307405.1 NZ_CP048344:c632886-632551 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT149144 NZ_CP063933:c2937009-2936788 [Klebsiella variicola]
ATGAGAACGGTTAACTATAGCGAAGCCCGACAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTATACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCGGTCATCATTAGTGCAGAAGAATTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATTATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CJ20

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References