Detailed information of TA system TA149135

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 102767..103094
• Replicon: plasmid p142_A-OXA181
• Accession: NZ_CP048338
• Organism: Escherichia coli strain 142

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: GZS12_RS24925
• Protein ID: WP_112047136.1
• Coordinates: 102767..102871 (-)
• Length: 35 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	102967..103094 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GZS12_RS24890	98128..98412	-	285	WP_001617873.1	type-F conjugative transfer system pilin chaperone TraQ	-
GZS12_RS24895	98539..98859	-	321	WP_001348757.1	conjugal transfer protein TrbA	-
GZS12_RS24900	98907..99604	-	698	Protein_121	IS1 family transposase	-
GZS12_RS24905	99719..100321	+	603	WP_000243712.1	transglycosylase SLT domain-containing protein	-
GZS12_RS24910	100618..101439	-	822	WP_162646450.1	DUF945 domain-containing protein	-
GZS12_RS24915	101558..101845	-	288	WP_000107535.1	hypothetical protein	-
GZS12_RS26125	101870..102076	-	207	WP_000275859.1	hypothetical protein	-
GZS12_RS24925	102767..102871	-	105	WP_112047136.1	Hok/Gef family protein	Toxin
GZS12_RS24930	102865..102981	-	117	WP_162646446.1	DUF5431 family protein	-
-	102967..103094	-	128	NuclAT_0	-	Antitoxin
-	102967..103094	-	128	NuclAT_0	-	Antitoxin
-	102967..103094	-	128	NuclAT_0	-	Antitoxin
-	102967..103094	-	128	NuclAT_0	-	Antitoxin
GZS12_RS24935	103121..104487	-	1367	Protein_128	IS3 family transposase	-
-	104533..104635	-	103	NuclAT_1	-	-
-	104533..104635	-	103	NuclAT_1	-	-
-	104533..104635	-	103	NuclAT_1	-	-
-	104533..104635	-	103	NuclAT_1	-	-
GZS12_RS24940	104647..105366	-	720	WP_001276232.1	plasmid SOS inhibition protein A	-
GZS12_RS24945	105363..105797	-	435	WP_000845940.1	conjugation system SOS inhibitor PsiB	-
GZS12_RS24950	105852..107810	-	1959	WP_000117179.1	ParB/RepB/Spo0J family partition protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaOXA-181 / qnrS1 / qnrB4 / blaDHA-1 / sul1 / mph(A) / catA1 / tet(B) / sitABCD	iucA / iucB / iucB / iucC / iucD / iutA	1..155486	155486
	-	inside	IScluster/Tn	-	-	98907..104487	5580

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-30)

Sequences

Toxin

Protein

• Length: 35 a.a.
• Molecular weight: 4056.73 Da
• Isoelectric Point: 9.0200

>T149135 WP_112047136.1 NZ_CP048338:c102871-102767 [Escherichia coli]
MLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 105 bp

>T149135 NZ_CP063932:c4595492-4595082 [Klebsiella variicola]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTCCACGGCGTTGTGGCGGC
GCTGGTGCTGTTGGTTCCCTGGCCGCTAAGCTACACCCCGATCTGGCTGCTGCTGTTGTCGCTGGTAGTGTTTGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGAGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAGATCCTCGGGACGCCGTGGGTCATCAATAGCGGTATGCTGCTGCGTTTACGCCATGTCGATACCCGGCGCGG
GCAGCATCTGTGGCTGGCGGCGGATAGCATGGATGCCGGAGAATGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin

Nucleotide

• Length: 128 bp

>AT149135 NZ_CP048338:c103094-102967 [Escherichia coli]
CGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGAAGATAGCCCCGTAGTAA
GTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin