Detailed information of TA system TA149132

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 24159..24398
• Replicon: plasmid p142_A-OXA181
• Accession: NZ_CP048338
• Organism: Escherichia coli strain 142

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: A0A762TWR7
• Locus tag: GZS12_RS24450
• Protein ID: WP_023144756.1
• Coordinates: 24264..24398 (+)
• Length: 45 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	24159..24219 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GZS12_RS24430	21689..22435	+	747	WP_000205718.1	conjugal transfer pilus acetylase TraX	-
GZS12_RS24435	22490..23050	+	561	WP_000139341.1	fertility inhibition protein FinO	-
GZS12_RS24440	23181..23393	+	213	WP_013023861.1	hypothetical protein	-
GZS12_RS24445	23906..24192	+	287	Protein_28	DUF2726 domain-containing protein	-
-	24159..24219	-	61	NuclAT_2	-	Antitoxin
-	24159..24219	-	61	NuclAT_2	-	Antitoxin
-	24159..24219	-	61	NuclAT_2	-	Antitoxin
-	24159..24219	-	61	NuclAT_2	-	Antitoxin
GZS12_RS24450	24264..24398	+	135	WP_023144756.1	type I toxin-antitoxin system Hok family toxin	Toxin
GZS12_RS24455	24695..24805	+	111	Protein_30	replication protein	-
GZS12_RS24460	24874..25656	-	783	WP_001300609.1	IS21-like element IS100kyp family helper ATPase IstB	-
GZS12_RS24465	25653..26675	-	1023	WP_000255944.1	IS21-like element IS100 family transposase	-
GZS12_RS24470	26756..26908	+	153	WP_072258247.1	replication protein A	-
GZS12_RS26115	27014..27148	+	135	Protein_34	protein CopA/IncA	-
GZS12_RS24475	27145..27219	+	75	WP_031943482.1	RepA leader peptide Tap	-
GZS12_RS24480	27212..28069	+	858	WP_000130640.1	incFII family plasmid replication initiator RepA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaOXA-181 / qnrS1 / qnrB4 / blaDHA-1 / sul1 / mph(A) / catA1 / tet(B) / sitABCD	iucA / iucB / iucB / iucC / iucD / iutA	1..155486	155486

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-44)

Sequences

Toxin

Protein

• Length: 45 a.a.
• Molecular weight: 4896.90 Da
• Isoelectric Point: 7.7209

>T149132 WP_023144756.1 NZ_CP048338:24264-24398 [Escherichia coli]
MTKYTLIGLLAVCATVLCFSLIFREQLCELNIHRGNTVVQVTLA

Nucleotide

• Length: 135 bp

>T149132 NZ_CP063932:2701729-2703051 [Klebsiella variicola]
ATGGCGACGCTTACCACCTGGATGAACAATGTGCGCGTGGGCACCTTAACCCGCCAGGCTAACGGGGCGCACAGTTTTCG
CTATGACGAAGAGTGGCTGCGCAGCCCGCGCGCCAGACCGCTCTCCCTCTCGCTGCCGCTGCAGTATGGCAATATCACCG
CCGACACGGTGTATCACTATTTTGATAACCTGCTACCCGACAGCCCGCAGGTCCGCGATCGGATCGTCAGGCGCTATCAG
GCGCGCTCGAAGCAGCCGTTCGATCTGCTGGCCGAAGTGGGACGGGATAGCGTAGGGGCGGTGACTCTGCTACCGCCGGG
GGAAGAGGCGCACCTCGAGGGGTTGCGCTGGCAGACGCTGGACGAGGCGCAGCTCACCGCTTTGCTGACCGCCTACCAGT
CGGATATCCCTCTCGGGATGATTACCGGCCAGGATGATTTCCGTATCTCGGTGGCCGGCGCCCAGGAGAAAACGGCGCTG
CTGCGGATGGGCGGGCACTGGTGTATTCCGCAGGGCACCACGCCGACCACGCATATCATTAAACTGCCCATCGGCGAGAT
AAAGCAGCCTAATGCGACGCTGGATCTGCGTGAAAGCGTGGATAACGAGTTTCTGTGCCTGGCGCTGGCCAGGGAACTGG
GCCTGGCGGTGCCGGAGGCAGAGATTATCACCACGCCGCGGATCCGCGCGCTGGCGGTGACCCGCTTTGACCGGCGCTGG
GCGCAGGAGGGGCGGGTGCTGCTGCGTCTGCCGCAGGAGGATCTGTGTCAGGCCTTTGGCATCCCGTCGTCGATGAAATA
TGAATCTGACGGCGGGCCGGGTATTGCGGCAATCATGGCCTTTCTGCTGGGCTCCAGTGAAGCCCTGAAAGATCGCGCAG
ACTTTATGAAATTCATGGTTTTTCAGTGGCTGGTTGGGGCAACGGACGGGCATGCGAAGAACTTCTCGCTCTTTTTGCTG
CCCGGTGGCAGCTACCGTCTAACGCCGTTCTACGACATCATTTCGGCGTTTCCGGTGCTGGGAGGCACGGGACTGCATCT
GCGCGACCTGAAGCTGTCGATGGGGCTCAATGCCACAAAAGGGCGGAAAACAGAGATTAACGCTATCTATCCGCGTCATT
TTCTGGCAACCGCCAAAGCGGTGAACTTTCCTGGGGAGCAGATGCTGGCGATCCTCCAGGAGTTTGCCGGGCATGTGCCG
CAGGCAATCGAAAGCGTTCGCCGGACGCTGCCGGCGGACTTTTCCACCCACGTCTGGCGGGCCATTACGGAAAATATGCT
GAAGCTGCACGCCCGCCTGCAGCAAGGGCTGCAGGCGTTATAG

Antitoxin

Nucleotide

• Length: 61 bp

>AT149132 NZ_CP048338:c24219-24159 [Escherichia coli]
TAAAGTGCATCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A762TWR7

Antitoxin