Detailed information of TA system TA149130

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4853654..4854256
• Replicon: chromosome
• Accession: NZ_CP048337
• Organism: Escherichia coli strain 142

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: GZS12_RS23250
• Protein ID: WP_000897305.1
• Coordinates: 4853945..4854256 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GZS12_RS23245
• Protein ID: WP_000356397.1
• Coordinates: 4853654..4853944 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GZS12_RS23225	4850156..4851058	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
GZS12_RS23230	4851055..4851690	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
GZS12_RS23235	4851687..4852616	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
GZS12_RS23240	4852831..4853049	-	219	WP_001298592.1	ribbon-helix-helix domain-containing protein	-
GZS12_RS23245	4853654..4853944	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
GZS12_RS23250	4853945..4854256	-	312	WP_000897305.1	hypothetical protein	Toxin
GZS12_RS23255	4854485..4855393	+	909	WP_001318161.1	alpha/beta hydrolase	-
GZS12_RS23260	4855457..4856398	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
GZS12_RS23265	4856443..4856880	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
GZS12_RS23270	4856877..4857749	-	873	WP_000920762.1	virulence factor BrkB family protein	-
GZS12_RS23275	4857743..4858342	-	600	WP_001314324.1	glucose-1-phosphatase	-
GZS12_RS23280	4858441..4859226	-	786	WP_000059679.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T149130 WP_000897305.1 NZ_CP048337:c4854256-4853945 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T149130 NZ_CP063932:c1173866-1173648 [Klebsiella variicola]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAACTGGCGGTATTTTACTCAGCCGCCGATCATCGTCTGGCTGAAC
TGACCATGAATAAGCTGTACGATAAGATCCCGACTTCAGTCTGGAAGTTTATCCGCTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT149130 WP_000356397.1 NZ_CP048337:c4853944-4853654 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT149130 NZ_CP063932:c1174267-1173893 [Klebsiella variicola]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACTTCGGCTGTCAATCTTCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCGCTTAATTATAAAATTAAGTACACTGAGGATAATAAACTGGTCGCTCAGGTTGACGAATATCTG
GACGATACTTTTACCTTGTTCAGTAACTACGGCATTAACTCAACGGATTTGCAAAAGTGGAAAAAATCCGGTAATCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTCTCGTGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7