Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3887087..3887781 Replicon chromosome
Accession NZ_CP048337
Organism Escherichia coli strain 142

Toxin (Protein)


Gene name yafO Uniprot ID Q0T7Q5
Locus tag GZS12_RS18515 Protein ID WP_001263491.1
Coordinates 3887087..3887485 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID L4JHF0
Locus tag GZS12_RS18520 Protein ID WP_000554755.1
Coordinates 3887488..3887781 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GZS12_RS18485 3882349..3883806 + 1458 WP_022645227.1 cytosol nonspecific dipeptidase -
GZS12_RS18490 3883815..3884096 + 282 WP_022645226.1 hypothetical protein -
GZS12_RS18495 3884113..3884622 - 510 WP_001361775.1 hydrolase -
GZS12_RS18500 3884684..3885298 - 615 WP_022645225.1 peptide chain release factor H -
GZS12_RS18505 3885295..3886434 - 1140 WP_022645224.1 RNA ligase RtcB family protein -
GZS12_RS18510 3886625..3887077 - 453 WP_023909048.1 GNAT family N-acetyltransferase -
GZS12_RS18515 3887087..3887485 - 399 WP_001263491.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
GZS12_RS18520 3887488..3887781 - 294 WP_000554755.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
GZS12_RS18525 3887833..3888888 - 1056 WP_022645222.1 DNA polymerase IV -
GZS12_RS18530 3888959..3889744 - 786 WP_072224360.1 putative lateral flagellar export/assembly protein LafU -
GZS12_RS18535 3889716..3891428 + 1713 Protein_3625 flagellar biosynthesis protein FlhA -
GZS12_RS18540 3891526..3892299 - 774 WP_022645219.1 C40 family peptidase -
GZS12_RS18545 3892485..3892745 + 261 WP_000729708.1 type II toxin-antitoxin system antitoxin DinJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15457.86 Da        Isoelectric Point: 8.0949

>T149123 WP_001263491.1 NZ_CP048337:c3887485-3887087 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T149123 NZ_CP063931:c82603-82133 [Klebsiella pneumoniae]
GTGATCCTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
TTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACGCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTGATCCAAAGCGCAAACATGGAT
GAATTACCGGGCAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAACTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11235.78 Da        Isoelectric Point: 5.2170

>AT149123 WP_000554755.1 NZ_CP048337:c3887781-3887488 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT149123 NZ_CP063931:c83043-82600 [Klebsiella pneumoniae]
ATGAAAACATTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
CGTAGCGCTTCTGGGCCAAATCAATGAAGGCCTGGACGGGAAGGTGGCGAATCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCGACCACGTTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGGATTATCCGCGTCATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAAGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAGACAGGAGCGTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TPK7

Antitoxin

Source ID Structure
AlphaFold DB A0A829G9H5

References