Detailed information of TA system TA149095

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 89048..89474
• Replicon: plasmid p010_A
• Accession: NZ_CP048331
• Organism: Escherichia coli strain 10

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: GZS13_RS24265
• Protein ID: WP_001312861.1
• Coordinates: 89048..89206 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	89250..89474 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GZS13_RS24230	84086..84313	-	228	WP_001254388.1	conjugal transfer relaxosome protein TraY	-
GZS13_RS24235	84407..85093	-	687	WP_000332487.1	PAS domain-containing protein	-
GZS13_RS24240	85287..85670	-	384	WP_001151564.1	relaxosome protein TraM	-
GZS13_RS24245	85956..86603	+	648	WP_000614282.1	transglycosylase SLT domain-containing protein	-
GZS13_RS24250	86900..87721	-	822	WP_001234469.1	DUF945 domain-containing protein	-
GZS13_RS24255	87842..88129	-	288	WP_000107537.1	hypothetical protein	-
GZS13_RS24265	89048..89206	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	89250..89474	-	225	NuclAT_0	-	Antitoxin
-	89250..89474	-	225	NuclAT_0	-	Antitoxin
-	89250..89474	-	225	NuclAT_0	-	Antitoxin
-	89250..89474	-	225	NuclAT_0	-	Antitoxin
GZS13_RS24270	89286..89474	+	189	WP_001299721.1	hypothetical protein	-
GZS13_RS24275	89486..90205	-	720	WP_001276234.1	plasmid SOS inhibition protein A	-
GZS13_RS24280	90202..90636	-	435	WP_000845901.1	conjugation system SOS inhibitor PsiB	-
GZS13_RS24285	90691..92649	-	1959	WP_001145469.1	ParB/RepB/Spo0J family partition protein	-
GZS13_RS24290	92708..92941	-	234	WP_000006004.1	DUF905 domain-containing protein	-
GZS13_RS24295	92997..93524	-	528	WP_000290793.1	single-stranded DNA-binding protein	-
GZS13_RS24305	93994..94242	-	249	WP_071606928.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aadA5 / qacE / sul1 / mph(A) / blaTEM-1B / sitABCD	-	1..129675	129675

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T149095 WP_001312861.1 NZ_CP048331:c89206-89048 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T149095 NZ_CP063927:c2873152-2872235 [Klebsiella pneumoniae]
ATGGATATACAGGCGCCTTATTATCGGCAGGTTGCTCTGCTAATGCAGGTTTTACCCTATGTTGCTGTCGAACGGGAATT
TGCGCTGAAAGGTGGCACTGCCATCAATCTCTTTATCCGCGATTTCCCTCGATTATCGGTGGATATCGACCTCGCCTGGG
TGCCCCTGGAAAGCAGAGCCATCGCTTTACCTCATATACGGGATGCGCTTGCACGTATTGCCGCCAATCTGCAACAACAG
GCAGGTATGAGCGCTGTATTACAAGCTAATCGTTCAGATGAGATGCGTGTCATTGTTACTACCGATAGCGCGCAAATAAA
GATAGAGGTTTCACCGGTCGCCCGGGGAACATTATATCCACCACAGGAGCGAGAAGTGGTGGGGGCGGTAGAGGACGTTT
TTGGCTATGCCAGCCTTCCAGTGGTTTCGTTGCCTGACCTCTACGGTGGAAAATTATGCGCGGCGCTGGATCGCCAGCAT
CCCAGAGATTTTTATGACGTGAAATTACTTCTTGATGCGCAGGAGCTTGACCGTCCCATTTTCAATGGGTTTATCGCGTA
TTTACTGAGCCATAATCGGCCTCTGGCTGAAGTGCTGAATCCTCGCTGGAAAGATATTGCTGAACCTTTTTACCGTGAAT
TCAGCGGGATGACATTTGAGACGATAGCCCTGGAGGAACTGACAGCAGTCCCCAACAGGATGATTGCTGCGTTAAAATCC
TGTTTCACACAACAGGATGTCGATTTTTTACTGTCATTTAAACGAGGCGAACCAGACTGGCGTTTAGCGCCGGAAATGCG
TATTCAGGATTTGCCTGCCGTGCAGTGGAAATTGCGCAATATTCATCAGATGCCAGCGATAAAACGCGCTGAGTCATTAG
ATAAACTGGAAAAGGTGCTGGCCGAGTGGCGCAGTTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT149095 NZ_CP048331:c89474-89250 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin