Detailed information of TA system TA149089

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3743851..3744545
• Replicon: chromosome
• Accession: NZ_CP048330
• Organism: Escherichia coli strain 10

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q47157
• Locus tag: GZS13_RS18315
• Protein ID: WP_001263489.1
• Coordinates: 3743851..3744249 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: GZS13_RS18320
• Protein ID: WP_000554758.1
• Coordinates: 3744252..3744545 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	3739439..3739519	-	81	NuclAT_12	-	-
-	3739439..3739519	-	81	NuclAT_12	-	-
-	3739439..3739519	-	81	NuclAT_12	-	-
-	3739439..3739519	-	81	NuclAT_12	-	-
GZS13_RS18290	3740115..3740573	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
GZS13_RS18295	3740834..3742291	+	1458	WP_113975472.1	cytosol nonspecific dipeptidase	-
GZS13_RS18300	3742348..3742869	-	522	Protein_3568	peptide chain release factor H	-
GZS13_RS18305	3742865..3743071	-	207	Protein_3569	RtcB family protein	-
GZS13_RS18310	3743389..3743841	-	453	WP_001059892.1	GNAT family N-acetyltransferase	-
GZS13_RS18315	3743851..3744249	-	399	WP_001263489.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
GZS13_RS18320	3744252..3744545	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
GZS13_RS18325	3744597..3745652	-	1056	WP_001226164.1	DNA polymerase IV	-
GZS13_RS18330	3745723..3746508	-	786	WP_000207552.1	putative lateral flagellar export/assembly protein LafU	-
GZS13_RS18335	3746480..3748192	+	1713	Protein_3575	flagellar biosynthesis protein FlhA	-
GZS13_RS18340	3748416..3748913	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	gmhA/lpcA	3743851..3759131	15280

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15487.84 Da
• Isoelectric Point: 7.4215

>T149089 WP_001263489.1 NZ_CP048330:c3744249-3743851 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T149089 NZ_CP063926:4488925-4489410 [Klebsiella variicola]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGACCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTCAT
GCCTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT149089 WP_000554758.1 NZ_CP048330:c3744545-3744252 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT149089 NZ_CP063926:4488635-4488928 [Klebsiella variicola]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGAACCGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Structures

Toxin

• AlphaFold DB: A0A090J8B1

Antitoxin

• AlphaFold DB: S1QAE3