Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 605541..606340 Replicon chromosome
Accession NZ_CP048330
Organism Escherichia coli strain 10

Toxin (Protein)


Gene name yhaV Uniprot ID A0A0A6SPA6
Locus tag GZS13_RS02895 Protein ID WP_000347275.1
Coordinates 605541..606005 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag GZS13_RS02900 Protein ID WP_001307405.1
Coordinates 606005..606340 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GZS13_RS02865 600542..600976 - 435 WP_000948824.1 PTS sugar transporter subunit IIA -
GZS13_RS02870 600994..601872 - 879 WP_001295548.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
GZS13_RS02875 601862..602641 - 780 WP_000406209.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
GZS13_RS02880 602652..603125 - 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
GZS13_RS02885 603148..604428 - 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
GZS13_RS02890 604677..605486 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
GZS13_RS02895 605541..606005 - 465 WP_000347275.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
GZS13_RS02900 606005..606340 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
GZS13_RS02905 606489..608060 - 1572 WP_001273752.1 galactarate dehydratase -
GZS13_RS02910 608435..609769 + 1335 WP_162656818.1 galactarate/glucarate/glycerate transporter GarP -
GZS13_RS02915 609785..610555 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17820.29 Da        Isoelectric Point: 9.8492

>T149071 WP_000347275.1 NZ_CP048330:c606005-605541 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKVPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T149071 NZ_CP063924:103056-103538 [Klebsiella pneumoniae]
GTGGGACGTATAACCGCGCCCGAGCCGTTATCCAGCTCTCATCAGCGGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACACAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGGACGA
AGCAAGTAGCTGGTTTTTACTCTCTGGCCACCGGTAGCGTTAACCATACGGAAGCTACAGGCAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTTCACGATGCCGTTCTACGCTGTTATCGCGTAGCTGAGAATATTGGCGTCCGTGCGATAATGGTTCACGCGCTTACTG
AAGAAGCTAAAGGCTTCTATACTCACCATGGATTTACGGCATCGCAAACTCATGAGCGGACTCTGTTCCTTAAACTTCCA
TGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT149071 WP_001307405.1 NZ_CP048330:c606340-606005 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT149071 NZ_CP063924:102802-103068 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCCGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATTGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A6SPA6

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References