Detailed information of TA system TA149070

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4555452..4556054
• Replicon: chromosome
• Accession: NZ_CP048326
• Organism: Escherichia coli strain 61

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: GZS14_RS21855
• Protein ID: WP_000897305.1
• Coordinates: 4555743..4556054 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GZS14_RS21850
• Protein ID: WP_000356397.1
• Coordinates: 4555452..4555742 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GZS14_RS21825	4551378..4552280	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
GZS14_RS21830	4552277..4552912	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
GZS14_RS21835	4552909..4553838	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
GZS14_RS21840	4554168..4554410	-	243	WP_001086388.1	hypothetical protein	-
GZS14_RS21845	4554629..4554847	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
GZS14_RS21850	4555452..4555742	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
GZS14_RS21855	4555743..4556054	-	312	WP_000897305.1	hypothetical protein	Toxin
GZS14_RS21860	4556283..4557191	+	909	WP_001162704.1	alpha/beta hydrolase	-
GZS14_RS21865	4557255..4558196	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
GZS14_RS21870	4558241..4558678	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
GZS14_RS21875	4558675..4559547	-	873	WP_000920762.1	virulence factor BrkB family protein	-
GZS14_RS21880	4559541..4560140	-	600	WP_086732335.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T149070 WP_000897305.1 NZ_CP048326:c4556054-4555743 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T149070 NZ_CP063924:94286-94702 [Klebsiella pneumoniae]
GTGAAAAAGACTTTTATGCTCGACACAAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGTGGCCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCCCCGCGCCATATCGAGCTGGTTGACGCGTTCTGTGCCCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGATGCGACCACGGAGATTAAGGTGGCCTTGCGACAGGCCGGGACGCCGATTGGCCCGAACGACACGGCGATAGC
CGGGCACGCCATCGCGGCCGGCGCCGTTCTGGTGACGAACAATGTAAGAGAGTTTGCGCGGGTCCCGGGCCTGGTGCTGG
AAGACTGGGTTAAGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT149070 WP_000356397.1 NZ_CP048326:c4555742-4555452 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT149070 NZ_CP063924:94059-94289 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAATGGCAATAACCGTGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAACGCGAGGACGTTGTCAGCGACGAAGGACGATTTGACCTGTGA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7