Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2877645..2878147 Replicon chromosome
Accession NZ_CP048326
Organism Escherichia coli strain 61

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag GZS14_RS14035 Protein ID WP_000767829.1
Coordinates 2877645..2877899 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag GZS14_RS14040 Protein ID WP_001259255.1
Coordinates 2877896..2878147 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GZS14_RS14005 2872660..2872887 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
GZS14_RS14010 2872901..2873959 - 1059 WP_000492321.1 transport protein YeeE -
GZS14_RS14015 2874138..2875496 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
GZS14_RS14020 2875486..2875548 - 63 WP_010723108.1 membrane protein YoeI -
GZS14_RS14025 2875763..2876692 - 930 WP_000803351.1 LysR family transcriptional regulator -
GZS14_RS14030 2876738..2877562 - 825 WP_000754749.1 SDR family oxidoreductase -
GZS14_RS14035 2877645..2877899 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
GZS14_RS14040 2877896..2878147 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
GZS14_RS14045 2878430..2878489 + 60 WP_032145667.1 his operon leader peptide -
GZS14_RS14050 2878635..2879534 + 900 WP_000131777.1 ATP phosphoribosyltransferase -
GZS14_RS14055 2879540..2880844 + 1305 WP_001614652.1 histidinol dehydrogenase -
GZS14_RS14060 2880841..2881911 + 1071 WP_000108939.1 histidinol-phosphate transaminase -
GZS14_RS14065 2881911..2882978 + 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - ugd / manB / manC / gnd / wcaJ / wcaG / gmd / wza / wzi / cpsACP / galF 2832404..2922543 90139


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T149064 WP_000767829.1 NZ_CP048326:c2877899-2877645 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T149064 NZ_CP063922:3588766-3589050 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTATGCAGAATCTGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT149064 WP_001259255.1 NZ_CP048326:c2878147-2877896 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT149064 NZ_CP063922:3588534-3588776 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References