Detailed information of TA system TA149048

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4556447..4557049
• Replicon: chromosome
• Accession: NZ_CP048322
• Organism: Escherichia coli strain 64

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: GZS15_RS21865
• Protein ID: WP_000897305.1
• Coordinates: 4556738..4557049 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GZS15_RS21860
• Protein ID: WP_000356397.1
• Coordinates: 4556447..4556737 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GZS15_RS21835	4552373..4553275	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
GZS15_RS21840	4553272..4553907	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
GZS15_RS21845	4553904..4554833	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
GZS15_RS21850	4555163..4555405	-	243	WP_001086388.1	hypothetical protein	-
GZS15_RS21855	4555624..4555842	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
GZS15_RS21860	4556447..4556737	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
GZS15_RS21865	4556738..4557049	-	312	WP_000897305.1	hypothetical protein	Toxin
GZS15_RS21870	4557278..4558186	+	909	WP_001162704.1	alpha/beta hydrolase	-
GZS15_RS21875	4558250..4559191	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
GZS15_RS21880	4559236..4559673	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
GZS15_RS21885	4559670..4560542	-	873	WP_000920762.1	virulence factor BrkB family protein	-
GZS15_RS21890	4560536..4561135	-	600	WP_086732335.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T149048 WP_000897305.1 NZ_CP048322:c4557049-4556738 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T149048 NZ_CP063921:920742-920960 [Klebsiella variicola]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAACTGGCGGTATTTTACTCAGCCGCCGATCATCGTCTGGCTGAAC
TGACCATGAATAAGCTGTACGATAAGATCCCGACTTCAGTCTGGAAGTTTATCCGCTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT149048 WP_000356397.1 NZ_CP048322:c4556737-4556447 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT149048 NZ_CP063921:920341-920715 [Klebsiella variicola]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACTTCGGCTGTCAATCTTCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCGCTTAATTATAAAATTAAGTACACTGAGGATAATAAACTGGTCGCTCAGGTTGACGAATATCTG
GACGATACTTTTACCTTGTTCAGTAACTACGGCATTAACTCAACGGATTTGCAAAAGTGGAAAAAATCCGGTAATCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTCTCGTGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7