Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 673581..674308 Replicon chromosome
Accession NZ_CP048322
Organism Escherichia coli strain 64

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag GZS15_RS03255 Protein ID WP_044804438.1
Coordinates 673581..673895 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag GZS15_RS03260 Protein ID WP_000560266.1
Coordinates 673892..674308 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GZS15_RS03235 669747..670733 - 987 WP_001387091.1 Gfo/Idh/MocA family oxidoreductase -
GZS15_RS03240 670812..671495 - 684 WP_001183042.1 vancomycin high temperature exclusion protein -
GZS15_RS03245 671572..672075 - 504 WP_001297151.1 M48 family metallopeptidase -
GZS15_RS03250 672160..673296 + 1137 WP_000018685.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
GZS15_RS03255 673581..673895 + 315 WP_044804438.1 type II toxin-antitoxin system toxin HigB Toxin
GZS15_RS03260 673892..674308 + 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
GZS15_RS03265 674353..676371 - 2019 WP_162676426.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
GZS15_RS03270 676697..679048 - 2352 WP_000695482.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12075.05 Da        Isoelectric Point: 10.0409

>T149028 WP_044804438.1 NZ_CP048322:673581-673895 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNAGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T149028 NZ_CP063917:3825225-3826547 [Klebsiella variicola]
ATGGCGACGCTTACCACCTGGATGAACAATGTGCGCGTGGGCACCTTAACCCGCCAGGCTAACGGGGCGCACAGTTTTCG
CTATGACGAAGAGTGGCTGCGCAGCCCGCGCGCCAGACCGCTCTCCCTCTCGCTGCCGCTGCAGTATGGCAATATCACCG
CCGACGCGGTGTATCACTATTTTGATAATCTGCTGCCCGACAGCCCGCAGGTCCGCGATCGGATCGTCAGGCGCTATCAG
GCGCGCTCGAAGCAGCCGTTCGATCTGCTGGCTGAAGTGGGACGGGATAGCGTAGGGGCGGTGACTCTGCTACCGCCGGG
GGAAGAGGCGCACCTCGAGGGGTTGCGCTGGCAGACGCTGGACGAGGCGCAGCTCACCGCTTTGCTGACCGCCTACCAGT
CGGATATCCCTCTCGGGATGATTACCGGCCAGGATGATTTCCGTATTTCGGTGGCCGGCGCCCAGGAGAAAACGGCGCTG
CTGCGGATGGGCGGGCACTGGTGTATTCCGCAGGGCACCACGCCGACCACGCATATCATTAAACTGCCCATCGGCGAGAT
AAAGCAGCCTAATGCGACGCTGGATCTGCGTGAAAGCGTGGATAACGAGTTTCTGTGCCTGGCGCTGGCCAGGGAACTGG
GCCTGGCGGTGCCGGAGGCAGAGATTATCACCACGCCGCGGATCCGCGCGCTGGCGGTGACCCGCTTTGACCGGCGCTGG
GCGCAGGAGGGACGGGTGCTGCTGCGTCTGCCGCAGGAGGATCTGTGTCAGGCCTTTGGCATCCCGTCGTCGATGAAATA
TGAATCTGACGGCGGGCCGGGTATTGCGGCAATCATGGCCTTTCTGCTGGGCTCCAGTGAAGCCCTGAAAGATCGCGCAG
ACTTTATGAAATTCATGGTCTTTCAGTGGCTGGTTGGGGCAACGGACGGGCATGCGAAGAACTTCTCGCTCTTTTTGCTG
CCCGGTGGCAGCTACCGTCTAACGCCGTTCTACGACATCATTTCGGCGTTTCCGGTGCTGGGAGGTACGGGACTGCATCT
GCGCGACCTGAAGCTGTCGATGGGGCTCAATGCCACAAAAGGGCGGAAAACAGAGATTAACGCCATCTATCCGCGTCATT
TTCTGGCAACCGCCAAAGCGGTGAACTTTCCTGGGGAGCTGATGCTGGCGATCCTCCAGGAGTTTGCCGGGCATGTGCCG
CAGGCAATCGAAAGCGCTCGCCGGACGCTGCCGGCGGACTTTTCCACCCACGTCTGGCGGGCCATTACGGAAAATATGCT
GAAGCTGCACGCCCGCCTGCAGCAAGGGCTGCAGGCGTTATAG

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT149028 WP_000560266.1 NZ_CP048322:673892-674308 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT149028 NZ_CP063917:3824959-3825225 [Klebsiella variicola]
ATGAATTCCCCGCAGATATACAGTCCCGTGCAGCTGGCCAATTATCTGAAGCTGCTGCGCCAGCAGAACCACTGGACCCA
GGATGCGCTGGCCAGGCGGATCGGCATTAAGCAGGCCACGCTGTCCAATTTTGAAAATCACCCCGACAAAACCACCTTAA
CCACGCTGTTTAAGATCCTCCAGTCGCTAGGGGTGGTGATGACGCTGAACGCCAAAGCCACAGAGACGGCGCCGGCTCCC
GATAACAACACGGACCTTGACTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References