Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 624325..625124 Replicon chromosome
Accession NZ_CP048322
Organism Escherichia coli strain 64

Toxin (Protein)


Gene name yhaV Uniprot ID F4VJD3
Locus tag GZS15_RS03005 Protein ID WP_000347266.1
Coordinates 624325..624789 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag GZS15_RS03010 Protein ID WP_001307405.1
Coordinates 624789..625124 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GZS15_RS02975 619326..619760 - 435 WP_000948824.1 PTS sugar transporter subunit IIA -
GZS15_RS02980 619778..620656 - 879 WP_001315856.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
GZS15_RS02985 620646..621425 - 780 WP_162676424.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
GZS15_RS02990 621436..621909 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
GZS15_RS02995 621932..623212 - 1281 WP_000681921.1 tagatose-bisphosphate aldolase subunit KbaZ -
GZS15_RS03000 623461..624270 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
GZS15_RS03005 624325..624789 - 465 WP_000347266.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
GZS15_RS03010 624789..625124 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
GZS15_RS03015 625273..626844 - 1572 WP_001273741.1 galactarate dehydratase -
GZS15_RS03020 627219..628553 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
GZS15_RS03025 628569..629339 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 624325..635780 11455


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17841.18 Da        Isoelectric Point: 9.4947

>T149027 WP_000347266.1 NZ_CP048322:c624789-624325 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRFFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T149027 NZ_CP063917:c2343367-2343149 [Klebsiella variicola]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAACTGGCGGTATTTTACTCAGCCGCCGATCATCGTCTGGCTGAAC
TGACCATGAATAAGCTGTACGATAAGATCCCGACCTCAGTCTGGAAGTTTATCCGCTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT149027 WP_001307405.1 NZ_CP048322:c625124-624789 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT149027 NZ_CP063917:c2343768-2343394 [Klebsiella variicola]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACTTCGGCTGTCAATCTTCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCGCTTAATTATAAAATTAAGTACACTGAGGATAATAAACTGGTCGCTCAGGTTGACGAATATCTG
GACGATACTTTTACCTTGTTCAGTAACTACGGCATTAACTCAACGGATTTGCAAAAGTGGAAAAAATCCGGTAATCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTCTCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A836NGD2

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References