Detailed information of TA system TA149023

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 6838..7102
• Replicon: plasmid p32-4_B
• Accession: NZ_CP048312
• Organism: Escherichia coli strain 32-4

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Z417
• Locus tag: GZS16_RS24250
• Protein ID: WP_001387489.1
• Coordinates: 6950..7102 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	6838..6898 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GZS16_RS24235	4060..4632	-	573	Protein_2	IS1 family transposase	-
GZS16_RS24240	4840..6102	-	1263	WP_000608644.1	IS1380-like element ISEc9 family transposase	-
GZS16_RS24245	6288..6635	-	348	Protein_4	protein finQ	-
-	6838..6898	-	61	NuclAT_0	-	Antitoxin
-	6838..6898	-	61	NuclAT_0	-	Antitoxin
-	6838..6898	-	61	NuclAT_0	-	Antitoxin
-	6838..6898	-	61	NuclAT_0	-	Antitoxin
GZS16_RS24250	6950..7102	+	153	WP_001387489.1	Hok/Gef family protein	Toxin
GZS16_RS24255	7174..7425	-	252	WP_001291964.1	hypothetical protein	-
GZS16_RS24260	7725..8021	+	297	WP_011264046.1	hypothetical protein	-
GZS16_RS25310	8086..8262	-	177	WP_001054897.1	hypothetical protein	-
GZS16_RS24265	8654..8863	+	210	WP_000062603.1	HEAT repeat domain-containing protein	-
GZS16_RS24270	8935..9597	-	663	WP_000644796.1	plasmid IncI1-type surface exclusion protein ExcA	-
GZS16_RS24275	9668..11422	-	1755	Protein_11	DotA/TraY family protein	-
GZS16_RS24280	11421..11537	-	117	Protein_12	IncI1-type conjugal transfer lipoprotein TraH	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCMY-42	-	1..69245	69245
	-	flank	IS/Tn	-	-	4840..6102	1262

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5761.10 Da
• Isoelectric Point: 8.7948

>T149023 WP_001387489.1 NZ_CP048312:6950-7102 [Escherichia coli]
MPQRTFLMMLIVVCVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T149023 NZ_CP063917:c699901-699554 [Klebsiella variicola]
ATGTGGGATGTAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCTTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTCCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTTTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGCGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Nucleotide

• Length: 61 bp

>AT149023 NZ_CP048312:c6898-6838 [Escherichia coli]
GAGGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: U9Z417

Antitoxin