Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3823422..3824116 Replicon chromosome
Accession NZ_CP048310
Organism Escherichia coli strain 32-4

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag GZS16_RS18335 Protein ID WP_001263489.1
Coordinates 3823422..3823820 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag GZS16_RS18340 Protein ID WP_000554758.1
Coordinates 3823823..3824116 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- 3819010..3819090 - 81 NuclAT_13 - -
- 3819010..3819090 - 81 NuclAT_13 - -
- 3819010..3819090 - 81 NuclAT_13 - -
- 3819010..3819090 - 81 NuclAT_13 - -
GZS16_RS18310 3819686..3820144 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
GZS16_RS18315 3820405..3821862 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
GZS16_RS18320 3821919..3822440 - 522 Protein_3586 peptide chain release factor H -
GZS16_RS18325 3822436..3822642 - 207 Protein_3587 RtcB family protein -
GZS16_RS18330 3822960..3823412 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
GZS16_RS18335 3823422..3823820 - 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
GZS16_RS18340 3823823..3824116 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
GZS16_RS18345 3824168..3825223 - 1056 WP_001226164.1 DNA polymerase IV -
GZS16_RS18350 3825294..3826079 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
GZS16_RS18355 3826051..3827763 + 1713 Protein_3593 flagellar biosynthesis protein FlhA -
GZS16_RS18360 3827987..3828484 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T149008 WP_001263489.1 NZ_CP048310:c3823820-3823422 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T149008 NZ_CP063915:c3633280-3632948 [Klebsiella variicola]
ATGGACAGAGGGGAAATCTGGCTTGTTTCACTGGATCCGATTGCTGGCCACGAGCAAAGTGGAAAACGTCCGGTCCTCAT
TGTATCGAAGGCATCGTTTAACAAGCTGACTCGGCTACCGGTCGTGGTTCCCGTCACCAGCGGCGGAAACTTTGCACGTA
CAGCAGGCTTCACGGTTTCACTGGAAGAGGCGGGGACCAAAACAACAGGCGTTATTCGCTGCGACCAGCCCAGAACTATC
GATATGGCGGCCCGGAACGGCAAGCGTCTGGAACGCATACCAGACGCCGTTGTCAATGAAGTGCTTGCCAGGCTGGATGC
GATTCTCAGTTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT149008 WP_000554758.1 NZ_CP048310:c3824116-3823823 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT149008 NZ_CP063915:c3633538-3633281 [Klebsiella variicola]
ATGTACAAAACTCGCCTGAAAAAGGTTGGCGGATCCATCATGCTGGCGGTTCCTCCCGCCGTGCTGAAAGCGCTGGAGCT
GTCGACAGACAGCGAAGTGGGTATGACTATAGATAATGGCTGCCTGATTATTGAACCGCAGAAACGGCCCCATTATTCGC
TTGAGGAACTGTTGGCGCAGTGCGATCCGCACGCCGAAATGAGCGAAGAGGATCGGGAATGGATTGATGCGCCTGCGGTG
GGCAAGGAGATTCTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References