Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 658251..659050 Replicon chromosome
Accession NZ_CP048310
Organism Escherichia coli strain 32-4

Toxin (Protein)


Gene name yhaV Uniprot ID D3GWU5
Locus tag GZS16_RS03175 Protein ID WP_000347272.1
Coordinates 658251..658715 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag GZS16_RS03180 Protein ID WP_001307405.1
Coordinates 658715..659050 (-) Length 112 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GZS16_RS03145 653252..653686 - 435 WP_000948824.1 PTS sugar transporter subunit IIA -
GZS16_RS03150 653704..654582 - 879 WP_001298314.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
GZS16_RS03155 654572..655351 - 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
GZS16_RS03160 655362..655835 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
GZS16_RS03165 655858..657138 - 1281 WP_001521382.1 tagatose-bisphosphate aldolase subunit KbaZ -
GZS16_RS03170 657387..658196 + 810 WP_000072171.1 aga operon transcriptional regulator AgaR -
GZS16_RS03175 658251..658715 - 465 WP_000347272.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
GZS16_RS03180 658715..659050 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
GZS16_RS03185 659199..660770 - 1572 WP_001273738.1 galactarate dehydratase -
GZS16_RS03190 661145..662479 + 1335 WP_000599651.1 galactarate/glucarate/glycerate transporter GarP -
GZS16_RS03195 662495..663265 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(12-150)

Antitoxin

(14-110)


Sequences


Toxin        


Download         Length: 155 a.a.        Molecular weight: 17832.26 Da        Isoelectric Point: 9.6924

>T148989 WP_000347272.1 NZ_CP048310:c658715-658251 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEEPH

Download         Length: 465 bp

>T148989 NZ_CP063913:c69146-68970 [Klebsiella pneumoniae]
GTGAAGCAAAGCGAGTTGAGGCGTTGGCTTGCAGCTCAAGGGGCAGAATTTAAAGATGGTACTAACCACTTGAAAATCTA
TCTCAACGGCAAGCAAACGGTAATGCCGAGACATCCGGGGAAGGAAATACCGGAACCGCTGAGGAAGGCAATTCTTAAGC
AACTCGGCATCAAATAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT148989 WP_001307405.1 NZ_CP048310:c659050-658715 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT148989 NZ_CP063913:c68922-68515 [Klebsiella pneumoniae]
ATGCGATACCCGGTAATTTTTGAGCATGACGAAACCGGCTGGGCGGTATTCTTTCCGGATATCCCAGAGGCAATGACAGG
AGGAGAGACCAGGGAGGAAGCGTTAGAAATGGCGCAAGATGCCTTGGTGACGGCGTTTGATTTTTACTTTGACGACCGCC
GGGAGATTCCCGCTCCATCAGCAGAAGGTGATGCGTTTGTTGAGGTTCCGGCCAGCGTGGCAGCTAAAGTATTGCTGTTA
AACCGTCTGGTCAGCACCAACACCAGCAATGCTGATCTCGCCCGCATGATTAACACGCGCCCACAGGAAGTGCAGCGCAT
CGTGTCTCTTGGTCACAGCACCAAAATCGATACGATCCAAAAAGCGCTATCAGCGTTGGGGCAAAAGATGGAAATTGTCG
TCCACTAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A829KUD2


Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References