Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2314067..2314569 Replicon chromosome
Accession NZ_CP048304
Organism Escherichia coli strain 9

Toxin (Protein)


Gene name yoeB Uniprot ID S1P977
Locus tag GZS17_RS10775 Protein ID WP_000767822.1
Coordinates 2314315..2314569 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag GZS17_RS10770 Protein ID WP_001259255.1
Coordinates 2314067..2314318 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GZS17_RS10745 2309245..2310312 - 1068 WP_000080122.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
GZS17_RS10750 2310312..2311382 - 1071 WP_000108995.1 histidinol-phosphate transaminase -
GZS17_RS10755 2311379..2312683 - 1305 WP_001297912.1 histidinol dehydrogenase -
GZS17_RS10760 2312689..2313588 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
GZS17_RS10765 2313734..2313784 - 51 WP_001364200.1 his operon leader peptide -
GZS17_RS10770 2314067..2314318 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
GZS17_RS10775 2314315..2314569 + 255 WP_000767822.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
GZS17_RS10780 2314652..2315476 + 825 WP_000754733.1 SDR family oxidoreductase -
GZS17_RS10785 2315522..2316451 + 930 WP_000803366.1 LysR family transcriptional regulator -
GZS17_RS10790 2316666..2316728 + 63 WP_010723108.1 membrane protein YoeI -
GZS17_RS10795 2316718..2318076 + 1359 WP_000019194.1 putrescine/proton symporter PlaP -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T148965 WP_000767822.1 NZ_CP048304:2314315-2314569 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINEIIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T148965 NZ_CP063910:3768161-3769078 [Klebsiella variicola]
ATGGATATACAGGCGCCCTATTATCGGCAGGTTGCTCTGTTAATGCAGGTTTTACCCTATGTTGCCGTCGAACGGGAATT
TGCGCTGAAAGGTGGCACGGCAATCAATCTCTTTATCCGTGATTTCCCACGATTATCGGTGGATATCGACCTCGCCTGGG
TGCCCCTGGAAAGCAGAACGATCGCTTTACCTCATATACGGGATGCGCTTGCACGTATTGCCGCCAATCTGCAACAACAG
GCAGGTATAAACGCTGTATTACAAGCTAATCGTTCCGATGAGATGCGTGTCGTTGTTACTACCGATAGCGCACAAATAAA
GATAGAGGTCTCACCGGTCGCACGGGGAACATTATATCCACCACAGGAGCGAGAGGTGGTGGGGGCGGTAGAGGACGTTT
TTGGCTATGCCAGCCTTCCGGTGGTTTCGTTGCCCGATCTCTACGGTGGAAAATTATGCGCGGCGCTGGATCGCCAGCAT
CCCAGAGATTTTTATGATGTGAAATTACTTCTTGATGCGCAGGAGCTTAACCGTCCCATTTTCAATGGGTTTATCGCGTA
TTTACTTAGCCATAATCGGCCTCTGGCTGAAGTGCTTAGTCCTCGCTGGAAAGATATTGCTGAACCCTTTTACCGTGAAT
TCAGCGGGATGACATTTGAGACGATAGCCCTGGAGGAACTGACAGCAGTCCCCTACAGGATGATTGATGCGTTAAAATCC
TGTTTCACGCAACAGGATATCGATTTTTTGCTGTCATTTAAACGAGGCGAACCAGACTGGCGTTTAGCGCCGGAAACACG
TATTCAGGATTTGCCTGCCGTGCAGTGGAAATTGCGCAATATTCATCAGATGCCAGCGATAAAACGCGCTGAGTCATTAG
ATAAACTGGAAAAGGTGTTGGCCGAGTGGCGCAGTTAG

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT148965 WP_001259255.1 NZ_CP048304:2314067-2314318 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT148965 NZ_CP063910:3767397-3768155 [Klebsiella variicola]
ATGCCATCAAAATTAAACTGGCTGCTGCAAAACAGTGCTCCCGGAGATGTGATCCTTCAGTCCTGGTTAAGCCGGCATGC
CATCTCTCCCTCTCTGGCCTTTAAATACACGCAAAGTGGCTGGTTAAAAAAACGAGGCAACGGCGTATACGCGCGTGCGG
GACGTGAGCCGGAGTGGAACGATGCATTAGCATGTTTGCAAAATCAGCTAGCCGCTCCGGTGTATGTCGCGGGGCTCAGT
AGCTTAGTCTGGCAGGGTCGTTCACACTATCTACAGCTCAAACAAAACCAGTGCTGGCTGAGTATGGAAAATAAAGCGCT
GTTGCCGAAATGGTTCAGGGAATTCCCGGGTGTGGAGTGGATAGTCATATCGGGGCAAAAACTGCCGGTGCTGGATGATA
GATACCGCGTACTGCTTGATGTAAAAGGGAAAAGACTCACCGGCAGTGCGCCGGAACTGGCTGCTTACGAGTTGTTGAGC
GCGGTACCGGGGACCCTCTCATTTAACCACGCGGCAGAGCTTTTCCAGGGACTGGTTAATCTTAATCCTCGTAAAGTAGA
GTATCTGCTTTCTGTCAGCCAGTCCGTGCAGACCAAGCGTTTGTATCTCTTTTTCGCCAGTTTTTACGAGCATGGTTGGC
TCAGACGAATCGATAGCCAAAAAATCGATCTGGGTGCGGGTAAGCGGCAAATCGTGGTAAATGGCAAGTTCAATGCTCAA
TATCAAATCACTGTGCCTGAACGCTTTCAAAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1P977

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References