Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1607924..1608651 Replicon chromosome
Accession NZ_CP048304
Organism Escherichia coli strain 9

Toxin (Protein)


Gene name higB Uniprot ID V0YLE2
Locus tag GZS17_RS07580 Protein ID WP_000547555.1
Coordinates 1607924..1608235 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A0H2V9Z2
Locus tag GZS17_RS07585 Protein ID WP_000126293.1
Coordinates 1608232..1608651 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GZS17_RS07550 1603065..1604774 + 1710 WP_001288134.1 formate hydrogenlyase subunit HycE -
GZS17_RS07555 1604784..1605326 + 543 WP_000493797.1 formate hydrogenlyase subunit HycF -
GZS17_RS07560 1605326..1606093 + 768 WP_000067399.1 formate hydrogenlyase subunit HycG -
GZS17_RS07565 1606090..1606500 + 411 WP_001291918.1 formate hydrogenlyase assembly protein HycH -
GZS17_RS07570 1606493..1606963 + 471 WP_000132956.1 hydrogenase maturation peptidase HycI -
GZS17_RS07575 1607006..1607761 + 756 WP_001305268.1 hypothetical protein -
GZS17_RS07580 1607924..1608235 + 312 WP_000547555.1 type II toxin-antitoxin system HigB family toxin Toxin
GZS17_RS07585 1608232..1608651 + 420 WP_000126293.1 helix-turn-helix domain-containing protein Antitoxin
GZS17_RS07590 1608743..1609171 - 429 WP_000536064.1 hypothetical protein -
GZS17_RS07595 1609556..1610083 + 528 WP_001078777.1 electron transport protein HydN -
GZS17_RS07600 1610236..1612488 + 2253 WP_001107659.1 carbamoyltransferase HypF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12439.18 Da        Isoelectric Point: 9.5146

>T148964 WP_000547555.1 NZ_CP048304:1607924-1608235 [Escherichia coli]
MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

>T148964 NZ_CP063910:3443863-3444246 [Klebsiella variicola]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGTACGCGAATGGCGCTAAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTGATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAACTACACCCTGAATAA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15426.46 Da        Isoelectric Point: 4.4596

>AT148964 WP_000126293.1 NZ_CP048304:1608232-1608651 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTIPHIKALSARFGVKPEWFL

Download         Length: 420 bp

>AT148964 NZ_CP063910:3443621-3443863 [Klebsiella variicola]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0YLE2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H2V9Z2

References