Detailed information of TA system TA148954

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 71527..72052
• Replicon: plasmid pRle248d
• Accession: NZ_CP048282
• Organism: Rhizobium leguminosarum bv. viciae 248

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: GYN07_RS28735
• Protein ID: WP_020052931.1
• Coordinates: 71780..72052 (+)
• Length: 91 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: A0A6N9ZA60
• Locus tag: GYN07_RS28730
• Protein ID: WP_017968209.1
• Coordinates: 71527..71778 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GYN07_RS28720	68056..69399	-	1344	WP_026265592.1	adenylate/guanylate cyclase domain-containing protein	-
GYN07_RS28725	70055..71392	+	1338	WP_020052930.1	nucleotide sugar dehydrogenase	-
GYN07_RS28730	71527..71778	+	252	WP_017968209.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
GYN07_RS28735	71780..72052	+	273	WP_020052931.1	Txe/YoeB family addiction module toxin	Toxin
GYN07_RS28740	72107..73333	-	1227	WP_026265593.1	DUF3095 domain-containing protein	-
GYN07_RS28745	73507..73986	+	480	WP_020052933.1	class I SAM-dependent methyltransferase	-
GYN07_RS28750	74011..75801	-	1791	WP_020052934.1	ABC transporter ATP-binding protein/permease	-
GYN07_RS28755	76193..76648	-	456	WP_020052935.1	Rrf2 family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..548709	548709

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-71)

Sequences

Toxin

Protein

• Length: 91 a.a.
• Molecular weight: 10795.52 Da
• Isoelectric Point: 8.4871

>T148954 WP_020052931.1 NZ_CP048282:71780-72052 [Rhizobium leguminosarum bv. viciae 248]
MKLLWTPNSWEEYEYWQRNDHKMVEKINELLMDTKRSPFKGLGKPEPLKGDLSGFWSRRILGEHRLVYCVTGKGPNQQLE
IILCLFHYEK

Nucleotide

• Length: 273 bp

>T148954 NZ_CP063908:4866049-4866582 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCTGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9273.53 Da
• Isoelectric Point: 5.6513

>AT148954 WP_017968209.1 NZ_CP048282:71527-71778 [Rhizobium leguminosarum bv. viciae 248]
MAHVRLTEFRQNIATYFDQVLSSRAPLLVTRQGSEAMVLVAEGEYESMQETLHLLSTPANSARLRESIGQLRKGQVVESD
PTL

Nucleotide

• Length: 252 bp

>AT148954 NZ_CP063908:4865772-4866038 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A6N9ZA60